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Lifileucel

Phase 3

Metastatic Melanoma | Monoclonal antibody | Oncology |Iovance Biotherapeutics, Inc.|Last Updated: Jun 26, 2026

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Trial Design
RandomizedCONTROLLEDDMC
Total Trials2
Total Enrollment890
FDA Designations
PRIORITY_REVIEWRMAT
Clinical trial landscape

Lifileucel · 5 trials · 10 indications

Phase 3 1Phase 2 3Phase 1 1
NCT05727904Study to Investigate Lifileucel Regimen Plus Pembrolizumab Compared With Pembrolizumab Alone in Participants With Untreated Advanced Melanoma.Metastatic Melanoma
RECRUITING670 Analytics
PHASE3RECRUITING
Study to Investigate Lifileucel Regimen Plus Pembrolizumab Compared With Pembrolizumab Alone in Participants With Untreated Advanced Melanoma.
Metastatic MelanomaUnlock trial analytics
Study Endpoints
Primary Endpoints
Objective Response Rate and Progression Free Survival
5 years total duration

To compare the efficacy of lifileucel plus pembrolizumab with the efficacy of pembrolizumab alone measured by objective response rate (ORR) and progression-free survival (PFS) assessed by the blinded independent review committee (BIRC) per Response Evaluation Criteria in Solid Tumors (RECIST) v1.1

Objective Response Rate
5 years

To evaluate the efficacy of lifileucel as measured by ORR per RECIST v1.1 as assessed by the IRC

Disease Assessment for Objective Response Rate
Every 6 weeks for 6 months, then every 3 months for a maximum of 60 months

Evaluate the efficacy of LN-144 in patients with unresectable or metastatic melanoma using the objective response rate (ORR), as assessed by the investigator per Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1 for Cohorts 1 \& 3 and as assessed by Independent Review Committee (IRC) per RECIST Version 1.1 for Cohorts 2 \& 4

Feasibility of neoadjuvant administration of MK-3475 and LN-144/Lifileucel in 3 stage IIIB-D melanoma patients (Phase 1)
Up to 2 years

Success rate of tumor infiltrating lymphocyte (TIL) expansion from all screened eligible patients who have had tumor-involved lymph node(s) removed; percentage of all eligible patients who have completed the screening and treated with LN-144/lifileucel.

Incidence of grade >= 3 treatment-emergent adverse events (both immune and non-immune related) (Phase 2)
Up to 2 years

The severity of AEs will be graded according to CTCAE version 5.0, and frequencies and percentages of patients with AEs will be tabulated by severity grade.

Secondary Endpoints
Overall Survival
7 years total duration
Complete Response Rate, Duration of Response and Event Free Survival
5 years total duration
Objective Response Rate, Progression Free Survival, Complete Response Rate, Duration of Responses, Event Free Survival and PFS2
5 years total duration
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Study Design & Arms
AllocationRANDOMIZED
MaskingNONE
ModelPARALLEL
PurposeTREATMENT
Treatment Arms
ArmTypeDescription
Arm AEXPERIMENTALLifileucel plus Pembrolizumab
Arm BACTIVE_COMPARATORPembrolizumab alone with Optional Crossover Period
Single ArmEXPERIMENTALLifileucel
Patients with Endometrial CancerEXPERIMENTAL -
Cohort 1EXPERIMENTALNon-cryopreserved LN-144 (Gen 1 infusion product) (Closed)
Cohort 2EXPERIMENTALCryopreserved lifileucel (LN-144) (Gen 2 infusion product) (Closed)
Cohort 3EXPERIMENTALRetreatment cohort: patients from Cohort 1, Cohort 2 or Cohort 4 may rescreen for a second TIL regimen therapy if they meet all Inclusion and Exclusion Criteria (except exclusion criterion b).
Cohort 4EXPERIMENTALCryopreserved lifileucel (LN-144) (Gen 2 infusion product)
Treatment (pembrolizumab, lifileucel)EXPERIMENTALPatients receive pembrolizumab IV on day -14, cyclophosphamide IV QD on days -7 to -6, fludarabine IV over 30 minutes QD on days -5 to -1, and lifileucel IV infusion on day 0. Patients also receive pembrolizumab IV on day 28 and 70, and undergo surgery on day 80. MAINTENANCE: Patients receive pembrolizumab IV every 6 weeks for up to 1 year in the absence of disease progression or unacceptable toxicity.
Interventions
NameTypeDescription
Lifileucel plus PembrolizumabBIOLOGICALA tumor sample is resected from each patient for Lifileucel manufacturing. Patients then receive Pembrolizumab followed by the lifileucel regimen which consists of non-myeloablative lymphodepletion, lifileucel infusion followed by IL-2. Patients will then continue to receive Pembrolizumab every 6 weeks until disease progression.
Pembrolizumab with Optional Crossover PeriodBIOLOGICALPatient will receive Pembrolizumab monotherapy every 6 weeks until disease progression. Upon verified confirmed disease progression by the blinded independent review central committee, patients may proceed to lifileucel monotherapy during an optional crossover period if they meet eligibility criteria. Optional crossover: A tumor sample is resected from each patient for Lifileucel manufacturing. Patients then receive the lifileucel regimen which consists of non-myeloablative lymphodepletion, lifileucel infusion followed by IL-2.
LifileucelBIOLOGICALA tumor sample is resected from each patient for lifileucel manufacturing. Patients will first receive the preparative non-myeloablative lymphodepletion (NMA-LD) regimen. They will then receive the lifileucel infusion, followed by an abbreviated course of aldesleukin.
CyclophosphamideDRUGGiven IV
FludarabineDRUGGiven IV
PembrolizumabBIOLOGICALGiven IV
Therapeutic Conventional SurgeryPROCEDUREUndergo surgery
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Eligibility Criteria
Age Range18 Years to 70 Years
SexALL
Healthy VolunteersNo
Study Sites81

Inclusion Criteria: 1. Participant has a histologically or pathologically confirmed diagnosis of Stage IIIC, IIID, or IV unresectable or metastatic melanoma. 2. In the investigator's assessment, the participant has an Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1 and an est...

Countries:United StatesAustraliaBelgiumCanadaFinlandFranceGermanyIsraelItalyNetherlandsSingaporeSouth KoreaSpainSwedenSwitzerlandUnited KingdomHungary
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Recent Changes (Last 90 Days)
LOWJun 26, 2026NCT05727904lastUpdatePostDate: changed
LOWJun 26, 2026NCT05727904lastUpdatePostDate: changed
LOWMay 26, 2026NCT06481592primaryCompletionDate: changed
LOWMay 26, 2026NCT05727904primaryCompletionDate: changed
LOWMay 26, 2026NCT05176470primaryCompletionDate: changed
MEDIUMMay 26, 2026NCT07288203Status: RECRUITING → ACTIVE_NOT_RECRUITING
LOWMay 24, 2026NCT05727904studyFirstPostDate: changed
LOWMay 24, 2026NCT06481592studyFirstPostDate: changed
LOWMay 24, 2026NCT07288203studyFirstPostDate: changed
LOWMay 24, 2026NCT05176470studyFirstPostDate: changed