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Lifileucel

Phase 3

Metastatic Melanoma | Monoclonal antibody | Oncology |Iovance Biotherapeutics, Inc.|Last Updated: Aug 3, 2026

Success Probability

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Trial Design

RandomizedCONTROLLEDDMC
Total Trials2
Total Enrollment890

FDA Designations

PRIORITY_REVIEWRMATFAST_TRACK

Clinical trial landscape

Lifileucel · 6 trials · 15 indications

Phase 3 1Phase 2 4Phase 1 1
NCT05727904Study to Investigate Lifileucel Regimen Plus Pembrolizumab Compared With Pembrolizumab Alone in Participants With Untreated Advanced Melanoma.Metastatic Melanoma
RECRUITING670 Analytics
PHASE3RECRUITING
Study to Investigate Lifileucel Regimen Plus Pembrolizumab Compared With Pembrolizumab Alone in Participants With Untreated Advanced Melanoma.
Metastatic MelanomaUnlock trial analytics

Study Endpoints

Primary Endpoints

Objective Response Rate and Progression Free Survival
5 years total duration

To compare the efficacy of lifileucel plus pembrolizumab with the efficacy of pembrolizumab alone measured by objective response rate (ORR) and progression-free survival (PFS) assessed by the blinded independent review committee (BIRC) per Response Evaluation Criteria in Solid Tumors (RECIST) v1.1

Objective Response Rate
5 years

To evaluate the efficacy of lifileucel as measured by ORR per RECIST v1.1 as assessed by the IRC

Disease Assessment for Objective Response Rate
Every 6 weeks for 6 months, then every 3 months for a maximum of 60 months

Evaluate the efficacy of LN-144 in patients with unresectable or metastatic melanoma using the objective response rate (ORR), as assessed by the investigator per Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1 for Cohorts 1 \& 3 and as assessed by Independent Review Committee (IRC) per RECIST Version 1.1 for Cohorts 2 \& 4

Feasibility of neoadjuvant administration of MK-3475 and LN-144/Lifileucel in 3 stage IIIB-D melanoma patients (Phase 1)
Up to 2 years

Success rate of tumor infiltrating lymphocyte (TIL) expansion from all screened eligible patients who have had tumor-involved lymph node(s) removed; percentage of all eligible patients who have completed the screening and treated with LN-144/lifileucel.

Incidence of grade >= 3 treatment-emergent adverse events (both immune and non-immune related) (Phase 2)
Up to 2 years

The severity of AEs will be graded according to CTCAE version 5.0, and frequencies and percentages of patients with AEs will be tabulated by severity grade.

Secondary Endpoints

Overall Survival
7 years total duration
Complete Response Rate, Duration of Response and Event Free Survival
5 years total duration
Objective Response Rate, Progression Free Survival, Complete Response Rate, Duration of Responses, Event Free Survival and PFS2
5 years total duration
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Study Design & Arms

AllocationRANDOMIZED
MaskingNONE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
Arm AEXPERIMENTALLifileucel plus Pembrolizumab
Arm BACTIVE_COMPARATORPembrolizumab alone with Optional Crossover Period
LifileucelEXPERIMENTAL -
Single ArmEXPERIMENTALLifileucel
Patients with Endometrial CancerEXPERIMENTAL -
Cohort 1EXPERIMENTALNon-cryopreserved LN-144 (Gen 1 infusion product) (Closed)
Cohort 2EXPERIMENTALCryopreserved lifileucel (LN-144) (Gen 2 infusion product) (Closed)
Cohort 3EXPERIMENTALRetreatment cohort: patients from Cohort 1, Cohort 2 or Cohort 4 may rescreen for a second TIL regimen therapy if they meet all Inclusion and Exclusion Criteria (except exclusion criterion b).
Cohort 4EXPERIMENTALCryopreserved lifileucel (LN-144) (Gen 2 infusion product)
Treatment (pembrolizumab, lifileucel)EXPERIMENTALPatients receive pembrolizumab IV on day -14, cyclophosphamide IV QD on days -7 to -6, fludarabine IV over 30 minutes QD on days -5 to -1, and lifileucel IV infusion on day 0. Patients also receive pembrolizumab IV on day 28 and 70, and undergo surgery on day 80. MAINTENANCE: Patients receive pembrolizumab IV every 6 weeks for up to 1 year in the absence of disease progression or unacceptable toxicity.

Interventions

NameTypeDescription
Lifileucel plus PembrolizumabBIOLOGICALA tumor sample is resected from each patient for Lifileucel manufacturing. Patients then receive Pembrolizumab followed by the lifileucel regimen which consists of non-myeloablative lymphodepletion, lifileucel infusion followed by IL-2. Patients will then continue to receive Pembrolizumab every 6 weeks until disease progression.
Pembrolizumab with Optional Crossover PeriodBIOLOGICALPatient will receive Pembrolizumab monotherapy every 6 weeks until disease progression. Upon verified confirmed disease progression by the blinded independent review central committee, patients may proceed to lifileucel monotherapy during an optional crossover period if they meet eligibility criteria. Optional crossover: A tumor sample is resected from each patient for Lifileucel manufacturing. Patients then receive the lifileucel regimen which consists of non-myeloablative lymphodepletion, lifileucel infusion followed by IL-2.
LifileucelBIOLOGICALStudy intervention will begin with a tumor resection from which lifileucel will be generated. The lifileucel regimen consists of a preparative NMA-LD regimen (ie, cyclophosphamide with mesna followed by fludarabine), the lifileucel infusion, and an abbreviated course of aldesleukin (interleukin-2).
CyclophosphamideDRUGGiven IV
FludarabineDRUGGiven IV
PembrolizumabBIOLOGICALGiven IV
Therapeutic Conventional SurgeryPROCEDUREUndergo surgery
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Eligibility Criteria

Age Range18 Years to 70 Years
SexALL
Healthy VolunteersNo
Study Sites81

Inclusion Criteria: 1. Participant has a histologically or pathologically confirmed diagnosis of Stage IIIC, IIID, or IV unresectable or metastatic melanoma. 2. In the investigator's assessment, the participant has an Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1 and an est...

Countries:United StatesAustraliaBelgiumCanadaFinlandFranceGermanyIsraelItalyNetherlandsSingaporeSouth KoreaSpainSwedenSwitzerlandUnited KingdomHungary
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Recent Changes (Last 90 Days)

LOWAug 3, 2026NCT07741877NEW_TRIAL: changed
LOWJun 26, 2026NCT05727904lastUpdatePostDate: changed
LOWJun 26, 2026NCT05727904lastUpdatePostDate: changed

Frequently asked questions about Lifileucel

What is Lifileucel used for?

Lifileucel is an investigational tumor-infiltrating lymphocyte therapy being studied for the treatment of multiple cancers, including metastatic melanoma, locally advanced melanoma, sarcoma, and endometrial cancer. It is a cell therapy being developed by Iovance Biotherapeutics for use in oncology.

How does Lifileucel work?

Lifileucel is a cell therapy that uses a patient's own tumor-infiltrating lymphocytes. These cells are collected from the tumor, expanded in the laboratory, and then infused back into the patient to attack cancer cells. It is being studied in combination with pembrolizumab in some trials.

Who makes Lifileucel?

Lifileucel is being developed by Iovance Biotherapeutics, Inc., a biopharmaceutical company traded on NASDAQ under the ticker symbol IOVA. The company is focused on developing cell therapies for cancer treatment.

What phase is Lifileucel in?

Lifileucel is in Phase 2 clinical development for multiple indications. It has received FDA designations including Priority Review, Regenerative Medicine Advanced Therapy (RMAT), and Fast Track. It is not yet approved and remains investigational.

What clinical trials is Lifileucel in?

Lifileucel is being studied in several clinical trials. NCT02360579 is a completed Phase 2 trial in metastatic melanoma with 220 participants. NCT05176470 is an active Phase 1 trial in locally advanced melanoma. NCT05727904 is a recruiting Phase 3 trial in advanced melanoma with 670 participants. NCT07741877 is a planned Phase 2 trial in soft tissue sarcoma.

Is Lifileucel the same as Lifileucel plus Pembrolizumab?

Lifileucel is sometimes referred to as Lifileucel plus Pembrolizumab when used in combination therapy. In clinical trials, Lifileucel is being studied both as a monotherapy and in combination with pembrolizumab, an immune checkpoint inhibitor, for the treatment of melanoma.