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Botensilimab

Phase 2

Metastatic Colorectal Cancer | Small molecule | Oncology |Agenus Inc.|Last Updated: Jun 11, 2026

Success Probability
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Market & Valuation
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Trial Design
RandomizedACTIVE_CONTROLLED
Total Trials1
Total Enrollment234
FDA Designations
FAST_TRACK
Clinical trial landscape

Botensilimab · 8 trials · 17 indications

Phase 2 6Phase 1 2
NCT06843551The Miami "EMPIRE" Trial - Eradication of Metastatic Pancreatic Cancer With Immuno-RadiationMetastatic Pancreatic Ductal Adenocarcinoma
RECRUITING20 Analytics
NCT06268015Botensilimab and Balstilimab Optimization in Colorectal CancerColorectal Cancer
ACTIVE NOT_RECRUITING16 Analytics
NCT06279130Pan-tumor Neoadjuvant Basket Study of Immune Check-point Inhibition and Novel Immuno-oncology CombinationsResectable MMR-deficient Solid Tumors
RECRUITING133 Analytics
NCT05630183A Study of Botensilimab in Participants With Metastatic Pancreatic CancerMetastatic Pancreatic Ductal Adenocarcinoma
COMPLETED81 Analytics
NCT05529316A Study of Botensilimab (AGEN1181) for the Treatment of Advanced MelanomaAdvanced Melanoma
ACTIVE NOT_RECRUITING150 Analytics
NCT05608044A Study of Botensilimab and Balstilimab for the Treatment of Colorectal CancerMetastatic Colorectal Cancer
ACTIVE NOT_RECRUITING234 Analytics
PHASE2RECRUITING
The Miami "EMPIRE" Trial - Eradication of Metastatic Pancreatic Cancer With Immuno-Radiation
Metastatic Pancreatic Ductal AdenocarcinomaUnlock trial analytics
PHASE2ACTIVE NOT_RECRUITING
Botensilimab and Balstilimab Optimization in Colorectal Cancer
Colorectal CancerUnlock trial analytics
PHASE2RECRUITING
Pan-tumor Neoadjuvant Basket Study of Immune Check-point Inhibition and Novel Immuno-oncology Combinations
Resectable MMR-deficient Solid TumorsUnlock trial analytics
PHASE2COMPLETED
A Study of Botensilimab in Participants With Metastatic Pancreatic Cancer
Metastatic Pancreatic Ductal AdenocarcinomaUnlock trial analytics
PHASE2ACTIVE NOT_RECRUITING
A Study of Botensilimab (AGEN1181) for the Treatment of Advanced Melanoma
Advanced MelanomaUnlock trial analytics
PHASE2ACTIVE NOT_RECRUITING
A Study of Botensilimab and Balstilimab for the Treatment of Colorectal Cancer
Metastatic Colorectal CancerUnlock trial analytics
Study Endpoints
Primary Endpoints
Clinical Benefit Rate (CBR)
Up to 15 months

The Clinical Benefit Rate (CBR) among participants will be reported. CBR is the number of participants achieving complete response (CR), partial response (PR) or stable disease after start of study therapy. Response will be assessed using Immune-related Response Evaluation Criteria in Solid Tumors (iRECIST) 1.1 criteria.

Disease control rate based on iRECIST at second restaging scan
24 weeks after screening

Disease control rate is defined as the proportion of subjects who have a complete response, partial response, or stable disease at the time of a second restaging scan. Complete response is defined as disappearance of all target lesions. Partial response is defined as at least a 30% decrease in the sum of diameters of target lesions, taking as reference the baseline sum diameters. Stable disease is defined as a decrease of less than 30% or an increase of less than 20%. Increases of 20% or greater must be confirmed 4-8 weeks later, and confirmation scans must also show an increase of at least 5 mm in the sum of lesion sizes or an increase in the number of lesions. Otherwise, the increase will be counted as stable disease.

Proportion of subjects with a best overall response of complete response or partial response according to iRECIST
up to 2 years

The number of subjects who went on treatment and had a complete response or partial response sometime during the study (prior to progression) divided by the number of subjects who went on treatment.

Major pathological response rate
Week 8

Percentage of patients in which a major pathologic response (MPR) is reported, defined as \<10% residual viable tumor (RVT) in the resection specimen.

Progression-free Survival as Assessed by Investigator
Up to 2 years

Progression-free survival will be defined as the time from the date of randomization to the date of the first objectively documented tumor progression, assessed by investigator per Response Evaluation Criteria in Solid Tumors Version 1.1 (RECIST 1.1), or death, whichever occurs first.

Objective Response Rate
First dose through up to 3 months

Objective response rate will be determined using Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST 1.1).

Maximum Tolerated Dose (MTD)
12 months

To determine the maximum tolerated dose (MTD) of botensilimab when given in combination with balstilimab + triplet chemotherapy regimen (consisting of nab-paclitaxel + gemcitabine + cisplatin) + chloroquine + celecoxib to be used in Part 2-Dose Expansion. MTD will be defined at the dose of botensilimab at which no more than 1 of 6 evaluable patients experiences a dose-limiting toxicity (DLT).

Safety and Tolerability of botensilimab in combination with balstilimab + triplet + chloroquine + celecoxib. Treatment-related toxicities as per National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0 (NCI-CTC AE V5.0).
End of Study (up to 2 years)

To evaluate the safety and tolerability of botensilimab in combination with balstilimab + triplet + chloroquine + celecoxib. Treatment-related toxicities as per National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0 (NCI-CTC AE V5.0).

Incidence Of Treatment-emergent Adverse Events (TEAEs)
First dose through 90 days following last study dose (up to 2 years)

TEAEs will include adverse events of special interest, immune-related adverse events, and adverse drug reactions, according to National Cancer Institute Common Terminology Criteria for Adverse Events (NCI CTCAE) Version 5.0.

DLT Of Botensilimab
First 28 days of treatment

DLTs will include any Grade 2 or greater drug related toxicity for all dose groups, according to NCI CTCAE version 5.0 and protocol specifications.

RP2D Of Botensilimab
First dose through 90 days following last study dose

MTD based on DLT occurrence at DLT period (28 days after first dose) and all TEAEs seen through 90 days following last study dose.

Secondary Endpoints
Objective Response Rate (ORR)
Up to 15 months
Progression-Free Survival (PFS)
Up to 5 years
Overall Survival (OS)
Up to 5 years
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Study Design & Arms
AllocationNA
MaskingNONE
ModelSINGLE_GROUP
PurposeTREATMENT
Treatment Arms
ArmTypeDescription
Radiation, Botensilimab Combined with Balstilimab Treatment GroupEXPERIMENTALParticipants in this group will receive Stereotactic Body Radiation Therapy (SBRT), followed by Botensilimab immunotherapy (ImT) for up to 24 weeks, in combination with Balstilimab ImT for up to one year. Total participation duration is up to five years.
TreatmentEXPERIMENTALbotensilimab + balstilimab until disease progression, then botensilimab + balstilimab + mFOLFOX6 (leucovorin, fluorouracil, oxaliplatin) + {bevacizumab or panitumumab}
Cohort 2: pMMR Safety run-in 1 - closed (accrual reached)EXPERIMENTAL5 Patients will be included and will be treated with 2 cycles of neoadjuvant immunotherapy consisting of 450mg balstilimab and 25mg botensilimab in Cycle 1 and 450mg balstilimab in Cycle 2 followed by surgery Accrual was reached in June 2024, cohort is closed.
Cohort 4: pMMR Safety run-in 2 - closed (accrual reached)EXPERIMENTAL5 Patients will be included and will be treated with 2 cycles of neoadjuvant immunotherapy consisting of 450mg balstilimab and 50mg botensilimab in Cycle 1 and 450mg balstilimab in Cycle 2 followed by surgery Accrual was reached in August 2024, cohort is closed.
Cohort 1: dMMR Safety run-in 1 - closed (accrual reached)EXPERIMENTAL5 Patients will be included and will be treated with 2 cycles of neoadjuvant immunotherapy consisting of 450mg balstilimab and 25mg botensilimab in Cycle 1 and 450mg balstilimab in Cycle 2 followed by surgery Accrual was reached in June 2025, cohort is closed.
Cohort 3: dMMR Safety run-in 2 - closed (accrual reached)EXPERIMENTAL5 Patients will be included and will be treated with 2 cycles of neoadjuvant immunotherapy consisting of 450mg balstilimab and 50mg botensilimab in Cycle 1 and 450mg balstilimab in Cycle 2 followed by surgery Acrrual was reached in August 2024, cohort is closed.
Cohort 6: dMMR Colorectal basket - closed (accrual reached)EXPERIMENTALPatients with resectable colon and rectal cancer will be treated with the regimen assesses as safe and feasible in the dMMR safety run-in. Treatment will be administered at day 1 and day 22 followed by surgery 8 weeks after registration. Accrual will be temporarily halted after accrual of the first 8 patients. If \>2 major pathological responses are observed accrual will continue to a total of 18 patients. Accrual was reached in July 2025, cohort is closed.
Cohort 5: dMMR Upper gastro-intestinal cancer basketEXPERIMENTALPatients with resectable oesophageal (adenocarcinoma), gastro-oesophageal junction, gastric and small bowel cancer will be treated with the regimen assessed as safe and feasible in the dMMR safety run-in. Treatment will be administered at day 1 and day 22 followed by surgery 8 weeks after registration. Accrual will be temporarily halted after accrual of the first 8 patients. If \>2 major pathological responses are observed accrual will continue to a total of 18 patients.
Cohort 7: dMMR "other cancers" basketEXPERIMENTALPatients with resectable solid tumors of various origins such as but not limited to breast-, prostate-, bladder cancer, Head\&Neck SCC, oesophageal SCC and sarcoma will be treated with the regimen assessed as safe and feasible in the dMMR safety run-in. Treatment will be administered at day 1 and day 22 followed by surgery 8 weeks after registration. Accrual will be temporarily halted after accrual of the first 8 patients. If \>2 major pathological responses are observed accrual will continue to a total of 18 patients.
Cohort 8: pMMR TNBC cohortEXPERIMENTALPatients with resectable Triple Negative Breast Cancer will be treated with the regimen assessed as safe and feasible in the pMMR safety run-in. Treatment will be administered at day 1 and day 22 followed by surgery 8 weeks after registration. Accrual will be temporarily halted after accrual of the first 8 patients. If \>2 major pathological responses are observed accrual will continue to a total of 18 patients.
Cohort 9: pMMR "other cancers" - cohort closedEXPERIMENTALPatients with resectable pMMR tumors of various origins will be treated with the regimen assessed as safe and feasible in the pMMR safety run-in. Treatment will be administered at day 1 and day 22 followed by surgery 8 weeks after registration. Accrual will be temporarily halted after accrual of the first 8 patients. If \>2 major pathological responses are observed accrual will continue to a total of 18 patients. Accrual was halted after 8 patients in July 2025, not more then 2 major pathological responses were observed, cohort remains closed.
Cohort 10: pMMR GEA basketEXPERIMENTALPatients with pMMR gastro-, esophageal or GE-junction tumors will receive study treatment that consists of cycle 1) botensilimab 50mg plus balstilimab 240mg 2) FLOT + bal 240mg 3) FLOT plus bot 50mg/bal 240mg 4-5) FLOT + bal 240mg. Surgery will take place a maximum of 6 weeks after start last treatment cycle. Patients will receive 8mg dexamethasone as premedication for docetaxel on the first day of infusion, and no additional doses of dexamethasone are allowed in the following days as an anti-emetic. Post-surgery, patients may receive 4 additional cycles of FLOT according to the standard of care. Evaluation will be performed after the first 3 patients are treated; if the proposed treatment is deemed feasible and all patients received the intended chemotherapy regimen, accrual may continue with the next 3 patients. If the first three patients undergo surgery according to plan without delays that are considered immune-related, accrual may continue beyond six patients (max 21).
Cohort 11: dMMR Rectal Organ Preservation basketEXPERIMENTALPatients with stage I-III dMMR rectal adenocarcinoma who prefer organ preservation are eligible. The study treatment will consist of 1 cycle of combination therapy on day 1: botensilimab 50mg plus balstilimab 450mg, followed by 2 cycles of balstilimab 450mg monotherapy on day 22 and day 43. If at the 1st response evaluation at 10 weeks a limited response, no response or progressive disease is observed, treatment according to standard of care, which may consist of direct surgery or additional neoadjuvant therapy may be considered after discussion in the MDT and with the study team.
Part 1: Combination (Safety Lead-in Phase)EXPERIMENTALParticipants will receive botensilimab in combination with standard-of-care chemotherapy (nab-paclitaxel + gemcitabine).
Part 2: CombinationEXPERIMENTALParticipants will receive botensilimab in combination standard-of-care chemotherapy (nab-paclitaxel + gemcitabine).
Part 2: Standard of CareACTIVE_COMPARATORParticipants will receive standard-of-care chemotherapy (nab-paclitaxel + gemcitabine).
Part 1 Cohort A: BotensilimabEXPERIMENTALParticipants refractory to PD-(L)1 therapy will receive botensilimab intravenously (IV).
Part 1 Cohort B: BotensilimabEXPERIMENTALParticipants refractory to PD-(L)1 and anti-CTLA-4 therapies will receive botensilimab IV.
Part 2 Cohort A: Botensilimab + BalstilimabEXPERIMENTALParticipants refractory to PD-(L)1 will receive botensilimab IV in combination with balstilimab IV.
Part 2 Cohort B: Botensilimab + BalstilimabEXPERIMENTALParticipants refractory to PD-(L)1 and CTLA-4 will receive botensilimab IV in combination with balstilimab IV.
Combination Botensilimab Dose 1 plus BalstilimabEXPERIMENTALParticipants will receive botensilimab at dose 1 given IV and balstilimab given IV.
Combination Botensilimab Dose 2 plus BalstilimabEXPERIMENTALParticipants will receive botensilimab at dose 2 given IV and balstilimab given IV.
Monotherapy Botensilimab Dose 1EXPERIMENTALParticipants will receive botensilimab dose 1 given IV.
Monotherapy Botensilimab Dose 2EXPERIMENTALParticipants will receive botensilimab dose 2 given IV.
Standard of CareACTIVE_COMPARATORParticipants will receive select standard of care as determined by the investigator.
DoseEscalationBotensilimab+balstilimab+nab-paclitaxel+gemcitabine+cisplatin +chloroquine + celecoxibEXPERIMENTALBotensilimab 50 mg IV, Balstilimab 240 mg, nab-paclitaxel 125 mg/m2 + gemcitabine 1000 mg/m2 +cisplatin 25 mg/m2; Chloroquine phosphate 500 mg po (300 mg equivalent chloroquine base); Celecoxib 200 mg po twice daily (BID) on Famotidine 20 mg po BID Aspirin 81 mg
Expansion Cohort-Botensilimab+balstilimab+nab-paclitaxel+gemcitabine+cisplatin+chloroquine+celecoxibEXPERIMENTALBotensilimab (MTD TBD), Balstilimab 240 mg IV, nab-paclitaxel 125 mg/m2 + gemcitabine 1000 mg/m2 +cisplatin 25 mg/m2 IV infusion ; Chloroquine phosphate 500 mg po (300 mg equivalent chloroquine base),Celecoxib 200 mg po twice daily (BID); Famotidine 20 mg po BID Aspirin 81 mg
3-Week MonotherapyEXPERIMENTAL3+3 Dose escalation: botensilimab, every 3 weeks, starting at dose level 0.1 milligrams/kilogram (mg/kg) up to 4 mg/kg, administered intravenously (IV) for up to 2 years.
6-Week MonotherapyEXPERIMENTAL3+3 Dose escalation: botensilimab, every 6 weeks, starting at dose level 1 mg/kg up to 4 mg/kg, administered IV for up to 2 years.
6-Week Combination TherapyEXPERIMENTAL3+3 Dose escalation: balstilimab, every 2 weeks, at dose level 3 mg/kg in combination with botensilimab, every 6 weeks, starting at dose level 0.1 mg/kg up to 4 mg/kg, administered IV for up to 2 years. Participants enrolled at sites in the United Kingdom (UK) may have the option for extended treatment. An additional cohort will investigate balstilimab, every 3 weeks, at 450 mg in combination with botensilimab every 6 weeks, at 150 mg, administered IV for up to 2 years.
Interventions
NameTypeDescription
Stereotactic Body Radiation TherapyRADIATIONThe radiation therapy (RT) prescription biologically effective dose (BED10) goal for tumor (α/β=10) aims to achieve at least BED10= 60 Gy for a single fraction plan and at least BED10=100 Gy for a multi-fraction plan. This equates to a prescription dose of at least 20 Gy in a single fraction, 42 Gy over 3 fractions, 50 Gy over 5 fractions, and 62 Gy over 10 fractions. Radiation therapy must be completed for up to 10 daily treatments within a 15-day course.
BotensilimabDRUGParticipants will be receive 75mg of Botensilimab immunotherapy administered via intravenous infusion, every six weeks for up to 4 doses, about 24 weeks. Botensilimab therapy will begin no later than seven (7) days after completion of radiation therapy.
BalstilimabDRUGParticipants will receive 240mg of Balstilimab immunotherapy, administered via intravenous infusion, every 2 weeks for up to one (1) year. Balstilimab therapy will begin no later than seven (7) days after completion of radiation therapy.
OxaliplatinDRUG85 mg/m2 IV every 2 weeks
LeucovorinDRUG400 mg/m2 IV every 2 weeks
FluorouracilDRUG400 mg/m2 IV bolus + 2,400 mg/m2 IV (over 46 hours) every 2 weeks
BevacizumabDRUG5 mg/kg IV every 2 weeks
PanitumumabDRUG6 mg/kg IV every 2 weeks
FLOT (Fluorouracil+Leucovorin+Oxaliplatin+Docetaxel)DRUG5-Fluorouracil (2400mg/m2), leucovorin (200mg/m2), oxaliplatin (85mg/m2), docetaxel (50mg/m2).
GemcitabineDRUGStandard-of-care chemotherapy administered intravenously.
Nab-paclitaxelDRUGStandard-of-care chemotherapy administered intravenously.
Standard of CareDRUGRegorafenib or trifluridine and tipiracil.
Chloroquine PhosphateDRUGan antimalarial agent, that is being used in this patient population to sensitize cancer cells to chemotherapy and leads to anticancer effects through inhibiting autophagy
CelecoxibDRUGa second-generation selective Cyclooxygenase 2 (COX-2) inhibitor, is being used in this patient population to enhance the therapeutic effect of cisplatin by inhibiting the expression of COX-2 as well as inhibit anti-apoptotic gene BCL-2 (B-cell lymphoma 2)
Nab paclitaxelDRUGmicrotubule inhibitor indicated for the treatment of Metastatic adenocarcinoma of the pancreas as first-line treatment, in combination with gemcitabine
CisplatinDRUGplatinum-based drug indicated for the treatment of advanced cancers
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Eligibility Criteria
Age Range18 Years to N/A
SexALL
Healthy VolunteersNo
Study Sites1

Inclusion Criteria: 1. ≥18 years old 2. Histologically or cytologically confirmed diagnosis of pancreatic ductal adenocarcinoma. 3. Microsatellite stable (MSS) disease by pathologic assessment. 4. Patients must have measurable disease as defined by RECIST 1.1. 5. Progression on ≥1 line of systemic ...

Countries:United StatesNetherlandsBelgiumBrazilFranceGermanyItalyRussiaSpainSwitzerlandUnited KingdomGeorgia
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Competitive Landscape -Colorectal Cancer 259 trials (matched to "Metastatic Colorectal Cancer")
Recent Changes (Last 90 Days)
MEDIUMJul 12, 2026NCT05630183TRIAL_REMOVED: changed
MEDIUMJul 12, 2026NCT05630183TRIAL_REMOVED: changed
MEDIUMJul 12, 2026NCT05630183TRIAL_REMOVED: changed
MEDIUMJul 12, 2026NCT05630183TRIAL_REMOVED: changed
HIGHJun 11, 2026NCT05630183Status: ACTIVE_NOT_RECRUITING → COMPLETED
HIGHJun 11, 2026NCT05630183Status: ACTIVE_NOT_RECRUITING → COMPLETED
LOWMay 26, 2026NCT06279130primaryCompletionDate: changed
LOWMay 26, 2026NCT06843551primaryCompletionDate: changed
LOWMay 26, 2026NCT05608044primaryCompletionDate: changed
LOWMay 26, 2026NCT05529316primaryCompletionDate: changed
LOWMay 26, 2026NCT06268015primaryCompletionDate: changed
LOWMay 26, 2026NCT06076837primaryCompletionDate: changed
LOWMay 26, 2026NCT05630183primaryCompletionDate: changed
LOWMay 26, 2026NCT03860272primaryCompletionDate: changed
LOWMay 24, 2026NCT06279130studyFirstPostDate: changed
LOWMay 24, 2026NCT06843551studyFirstPostDate: changed
LOWMay 24, 2026NCT03860272studyFirstPostDate: changed
LOWMay 24, 2026NCT05529316studyFirstPostDate: changed
LOWMay 24, 2026NCT06076837studyFirstPostDate: changed
LOWMay 24, 2026NCT06268015studyFirstPostDate: changed