Full Press Release Details
Proteoform Analysis
Nautilus Biotechnology
October 3, 2024
In our Translating Proteomics episode titled “ Harnessing Proteoforms to Understand Life’s Complexity “ , Parag and Andreas discussed why proteoforms are important in a theoretical sense. In this episode, Parag sits down with Northwestern University Professor and proteoform pioneer, Neil Kelleher to dive deep into the biology of proteoforms. They cover:
• What proteoforms are
• Examples of the importance of proteoforms
• The scale of and technological advances needed to meet the challenges of proteoform biology
Find this episode on YouTube , Apple Podcasts , and Spotify .
Browse all episodes of Translating Proteomics.
Translating Proteomics Ep 9 - Are Proteins a Myth?
Stream the episode on YouTube
Chapters
• 00:00 – Introduction to proteoforms
• 03:36 – Why the term “proteoform” was coined
• 11:55 – Are proteins a myth?
• 13:39 – Evidence for the importance of proteoforms
• 22:45 – The Human Proteoform Atlas and Project
• 30:09 – How many proteoforms are there?
• 33:32 – Technological developments required for routine proteoform analysis
• 41:45 – Takeaways with Parag and Andreas
Examples of the power of proteoforms covered in this episode
• Recent work from Neil’s lab showing blood proteoforms can help predict liver transplant success ( Melani et al. 2022) .
• Work form Ying Ge’s lab showing changes in troponin proteoforms correlate with varying degrees of heart disease ( Zhang et al. 2011) .
• The BioTyper – a mass spectrometry-based device that can identify different kinds of microbes.
Additional proteoform resources
• The Human Proteoform Atlas webpage
• Publication describing The Human Proteoform Atlas (Hollas et al. 2022)
• Publication discussing how many human proteoforms there are (Aebersold et al. 2018)
• Animation – Proteoform Analysis on the Nautilus Proteome Analysis Platform
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