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GSK3228836

Phase 2

Hepatitis B | Small molecule | Infectious Disease |GSK plc|Last Updated: Dec 30, 2025

Success Probability

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Trial Design

RandomizedCONTROLLEDDMC
Total Trials4
Total Enrollment601

FDA Designations

No designations recorded

Clinical trial landscape

GSK3228836 · 5 trials · 2 indications

Phase 2 4Phase 1 1
NCT05276297A Study on the Safety, Efficacy and Immune Response Following Sequential Treatment With an Anti-sense Oligonucleotide Against Chronic Hepatitis B (CHB) and Chronic Hepatitis B Targeted Immunotherapy (CHB-TI) in CHB Patients Receiving Nucleos(t)Ide Analogue (NA) TherapyHepatitis B, Chronic
COMPLETED174 Analytics
NCT04676724Study of Sequential GSK3228836 and Peginterferon Treatment in Participants With Chronic Hepatitis B (CHB)Hepatitis B
COMPLETED108 Analytics
NCT04544956A Mechanistic Study of GSK3228836 With Fine Needle Aspiration (FNA) in Participants With Chronic Hepatitis BHepatitis B
COMPLETED12 Analytics
NCT04449029A Study of GSK3228836 in Participants With Chronic Hepatitis B (CHB)Hepatitis B
COMPLETED457 Analytics
PHASE2COMPLETED
A Study on the Safety, Efficacy and Immune Response Following Sequential Treatment With an Anti-sense Oligonucleotide Against Chronic Hepatitis B (CHB) and Chronic Hepatitis B Targeted Immunotherapy (CHB-TI) in CHB Patients Receiving Nucleos(t)Ide Analogue (NA) Therapy
Hepatitis B, ChronicUnlock trial analytics
PHASE2COMPLETED
Study of Sequential GSK3228836 and Peginterferon Treatment in Participants With Chronic Hepatitis B (CHB)
Hepatitis BUnlock trial analytics
PHASE2COMPLETED
A Mechanistic Study of GSK3228836 With Fine Needle Aspiration (FNA) in Participants With Chronic Hepatitis B
Hepatitis BUnlock trial analytics
PHASE2COMPLETED
A Study of GSK3228836 in Participants With Chronic Hepatitis B (CHB)
Hepatitis BUnlock trial analytics

Study Endpoints

Primary Endpoints

Percentage of participants reporting any grade 3 adverse event (AE) from first dose of GSK3228836 up to study end
From first dose of GSK3228836 (Treatment 1 -Day 1) up to study end (Treatment 2-Day 505/Day 673/Day 841)

An AE is any untoward medical occurrence (an unfavourable/unintended sign - including an abnormal laboratory finding), symptom, or disease (new or exacerbated) in a clinical study participant that is temporally associated with the study intervention. A grade 3 AE is an AE which prevents normal, everyday activities (in adults, such an AE would, for example, prevent attendance at work/school and would necessitate the administration of corrective therapy).

Percentage of participants reporting any serious adverse event (SAE) from first dose of GSK3228836 up to study end
From first dose of GSK3228836 (Treatment 1-Day 1) up to study end (Treatment 2-Day 505/Day 673/Day 841)

An SAE is any untoward medical occurrence that results in death, is life-threatening, requires hospitalization or prolongation of existing hospitalization, results in disability/incapacity, is a congenital anomaly/birth defect in the offspring of a study participant or is an abnormal pregnancy outcome.

Percentage of participants reporting any adverse events of special interest (AESIs) grade 3 or higher from first dose of GSK3228836 up to study end
From first dose of GSK3228836 (Treatment 1-Day 1) up to study end (Treatment 2-Day 505/Day 673/Day 841)

AESI related to GSK3228836 treatment include thrombocytopenia, alanine transaminase (ALT) increases, vascular inflammation and complement activation, renal injury or injection site reactions. AESI related to GSK3528869A include liver disease-related (LDR) AEs, hematological AESI or potential immune-mediated diseases (pIMDs). A grade 3 AE is an AE which prevents normal, everyday activities (in adults, such an AE would, for example, prevent attendance at work/school and would necessitate the administration of corrective therapy).

Percentage of participants who achieve sustained virologic response (SVR) for 24 weeks after the planned end of active treatment in the absence of rescue medication, and difference between treatment arms (corresponding to GSK3228836 regimens)
For up to 24 weeks after the planned end of active treatment (planned end of active treatment = Treatment 1-Day 78 for ASO12 group, Treatment 1-Day 162 for ASO24 group and Treatment 2-Day 169 for ASO12-TI and ASO24-TI groups)

SVR is defined as Hepatitis B surface antigen (HBsAg) below (\<) lower limit of quantification (LLOQ) and HBV deoxyribose nucleic acid (DNA) \< LLOQ. Rescue medication is defined as any medication initiated for the purpose of antiviral suppression other than the background stable NA therapy irrespective of the reason.

Treatment Arm 1 - Percentage of Participants Achieving Sustained Virologic Response (SVR) for 24 Weeks After End of Treatment
Up to 24 weeks off treatment (Study Weeks 48 to 72)

Sustained virologic response is defined as undetectable levels of Hepatitis B surface antigen (HBsAg) and Hepatitis-B virus deoxy-ribonucleic acid (HBV DNA) on treatment. The SVR was a composite endpoint defined as HBsAg and HBV DNA levels were less than (\<) Lower limit of quantitation (LLOQ) at the planned end of sequential treatment of GSK3228836 and PegIFN treatment which is sustained for 24 weeks post-GSK3228836 and PegIFN treatment in the absence of any rescue medication. Percentage values are rounded-off.

Treatment Arm 2 - Percentage of Participants Achieving Sustained Virologic Response (SVR) for 24 Weeks After End of Treatment
Up to 24 weeks off treatment (Study Weeks 36 to 60)

Sustained virologic response is defined as undetectable levels of Hepatitis B surface antigen (HBsAg) and Hepatitis-B virus deoxy-ribonucleic acid (HBV DNA) on treatment. The SVR was a composite endpoint defined as HBsAg and HBV DNA levels were less than (\<) Lower limit of quantitation (LLOQ) at the planned end of sequential treatment of GSK3228836 and PegIFN treatment which is sustained for 24 weeks post-GSK3228836 and PegIFN treatment in the absence of any rescue medication. Percentage values are rounded-off.

Percentage of Participants Achieving Serum Hepatitis B Virus Surface Antigen (HBsAg) Level Less Than (<) Lower Limit of Quantitation (LLOQ)
Up to Week 12

Percentage of participants achieving serum HBsAg level \<LLOQ were reported. Percentage values are rounded-off.

Number of Participants Achieving Sustained Virologic Response (SVR)
Up to Week 48

The SVR was a composite endpoint defined as Hepatitis B surface antigen (HBsAg) and Hepatitis B virus (HBV) Deoxyribonucleic acid (DNA) levels were less than (\<) Lower limit of quantitation (LLOQ) at the planned end of GSK3228836 treatment which is sustained for 24 weeks post-GSK3228836 treatment in the absence of rescue medication.

Area under the concentration-time curve (AUC) from time zero (pre-dose) extrapolated to infinite time [AUC(0-infinity)]
Up to Day 50 post-dose
Maximum observed concentration (Cmax)
Up to Day 50 post-dose

Secondary Endpoints

Percentage of participants reporting each solicited administration site event post-GSK3528869A study intervention administration
Within 7 days post-administration (day of administration + 6 subsequent days) of each dose of GSK3528869A study intervention
Percentage of participants reporting each solicited systemic event post-GSK3528869A study intervention administration
Within 7 days post-administration (day of administration + 6 subsequent days) of each dose of GSK3528869A study intervention
Percentage of participants reporting any unsolicited AE post-GSK3528869A study intervention administration
Within 30 days post-administration (day of administration + 29 subsequent days) of each dose of GSK3528869A study intervention
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Study Design & Arms

AllocationRANDOMIZED
MaskingSINGLE
ModelSEQUENTIAL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
ASO24-TI GroupEXPERIMENTALEligible participants receive GSK3228836 (Treatment 1) study intervention for 24 weeks of Treatment 1 period, followed by GSK3528869A (Treatment 2) study intervention administered at Days 1, 57, 113 and 169 of Treatment 2 period. The interval between Treatment 1 and Treatment 2 is preferably 1 week, with the possibility to extend up to 12 weeks.
ASO24 GroupACTIVE_COMPARATOREligible participants receive GSK3228836 (Treatment 1) study intervention for 24 weeks of Treatment 1 period, followed by non-active control (Treatment 2) study intervention administered at Days 1, 57, 113 and 169 of Treatment 2 period. The interval between Treatment 1 and Treatment 2 is preferably 1 week, with the possibility to extend up to 12 weeks.
ASO12-TI GroupEXPERIMENTALEligible participants receive GSK3228836 (Treatment 1) study intervention for 12 weeks of Treatment 1 period, followed by GSK3528869A (Treatment 2) study intervention administered at Days 1, 57, 113 and 169 of Treatment 2 period. The interval between Treatment 1 and Treatment 2 is preferably 1 week, with the possibility to extend up to 12 weeks.
ASO12 GroupACTIVE_COMPARATOREligible participants receive GSK3228836 (Treatment 1) study intervention for 12 weeks of Treatment 1 period, followed by non-active control (Treatment 2) study intervention administered at Days 1, 57, 113 and 169 of Treatment 2 period. The interval between Treatment 1 and Treatment 2 is preferably 1 week, with the possibility to extend up to 12 weeks.
GSK3228836 300 mg (24 weeks) + PegIFN 180 mcg (24 weeks)EXPERIMENTALParticipants on stable NA therapy received 300 milligrams per week (mg/week) GSK3228836 for 24 weeks (plus a loading dose on Day 4 and 11), followed by Pegylated Interferon (PegIFN) 180 microgram per week (mcg/week) up to 24 weeks.
GSK3228836 300 mg (12 weeks) + PegIFN 180 mcg (24 weeks)EXPERIMENTALParticipants on stable NA therapy received 300mg/week GSK3228836 for 12 weeks (plus a loading dose on Day 4 and 11), followed by PegIFN 180 mcg/week up to 24 weeks.
GSK3228836 300 mgEXPERIMENTALParticipants on stable nucleos(t)ide therapy will receive GSK3228836 300 mg subcutaneously (SC) weekly once for 12 weeks along with a loading dose of GSK3228836 300 mg in Week 1 (Day 4) and Week 2 (Day 11).
Cohort 1: GSK3228836 300 mg + LDEXPERIMENTALEligible participants on stable nucleos(t)ide treatment will receive 300 milligrams (mg) GSK3228836 once weekly for 24 weeks along with loading dose (LD) of 300 mg GSK3228836 on Day 4 and Day 11.
Cohort 1: GSK3228836 300 mg + LD/ GSK3228836 150 mg + PlaceboEXPERIMENTALEligible participants on stable nucleos(t)ide treatment will receive 300 mg GSK3228836 once weekly for 12 weeks along with LD of 300 mg GSK3228836 on Day 4 and Day 11 followed by step-down in dose of 150 mg GSK3228836 once weekly for 12 weeks along with placebo to match to maintain participant blinding.
Cohort 1: GSK3228836 300 mg + LD/ PlaceboEXPERIMENTALEligible participants on stable nucleos(t)ide treatment will receive 300 mg GSK3228836 once weekly for 12 weeks along with LD of 300 mg GSK3228836 on Day 4 and Day 11 followed by placebo once weekly for 12 weeks.
Cohort 1: Placebo/ GSK3228836 300 mg + Placebo LDEXPERIMENTALEligible participants on stable nucleos(t)ide treatment will receive placebo once weekly for 12 weeks followed by 300 mg GSK3228836 once weekly for 12 weeks along with placebo LD to match on Day 4 and Day 11.
Cohort 2: GSK3228836 300 mg + LDEXPERIMENTALEligible participants not currently on nucleos(t)ide therapy will receive 300 mg GSK3228836 once weekly for 24 weeks along with LD of 300 mg GSK3228836 on Day 4 and Day 11.
Cohort 2: GSK3228836 300 mg + LD/ GSK3228836 150 mg + PlaceboEXPERIMENTALEligible participants not currently on nucleos(t)ide therapy will receive 300 mg GSK3228836 once weekly for 12 weeks along with LD of 300 mg GSK3228836 on Day 4 and Day 11 followed by step-down in dose of 150 mg GSK3228836 once weekly for 12 weeks along with placebo to match to maintain participant blinding.
Cohort 2: GSK3228836 300 mg + LD/ PlaceboEXPERIMENTALEligible participants not currently on nucleos(t)ide therapy will receive 300 mg GSK3228836 once weekly for 12 weeks along with LD of 300 mg GSK3228836 on Day 4 and Day 11 followed by placebo once weekly for 12 weeks.
Cohort 2: Placebo/ GSK3228836 300 mg + Placebo LDEXPERIMENTALEligible participants not currently on nucleos(t)ide therapy will receive placebo once weekly for 12 weeks followed by 300 mg GSK3228836 once weekly for 12 weeks along with placebo LD to match on Day 4 and Day 11.
Participants with Moderate (CP-B) hepatic impairmentEXPERIMENTAL -
Participants with Mild (CP-A) hepatic impairmentEXPERIMENTAL -
Healthy participantsEXPERIMENTAL -

Interventions

NameTypeDescription
GSK3228836DRUG2 doses of GSK3228836 study intervention administered subcutaneously once per week for 12 weeks (Day 1 up to Day 78) to participants in ASO12-TI and ASO12 groups, or for 24 weeks (Day 1 up to Day 162) to participants in ASO24-TI and ASO24 groups, plus loading doses administered at Day 4 and Day 11 (2 doses each day) to participants in all groups during Treatment 1 period.
GSK3528869ABIOLOGICALThe GSK3528869A chronic Hepatitis B targeted immunotherapy (CHB-TI) consisting of 4 doses administered intramuscularly as follows: * 1 dose of the Chimpanzee adenovectored HBV vaccine (ChAd155-hIi-HBV) at Day 1 of Treatment 2 period. * 1 dose of the Modified Vaccinia Virus Ankara HBV vaccine (MVA-HBV) at Day 57 of Treatment 2 period. * 2 subsequent doses of the AS01B-4-adjuvanted HBc-HBs proteins (HBc-HBs/AS01B-4) administered at Day 113 and Day 169 of Treatment 2 period.
ControlDRUG4 doses of non-active control administered intramuscularly in the deltoid region of the non-dominant arm at Days 1, 57, 113 and 169 to participants in ASO24 and ASO12 control groups during Treatment 2 period.
PegIFNDRUGParticipants will be administered PegIFN.
NA therapyDRUGParticipants will continue to receive their NA therapy for the duration of the study.
Nucleos(t)ide therapyDRUGParticipants receiving nucleos(t)ide therapy upon entry in the study will continue to receive nucleos(t)ide therapy for the duration of the study.
PlaceboDRUGPlacebo will be available as a clear colorless solution for injection to be administered subcutaneously once weekly.
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Eligibility Criteria

Age Range18 Years to 65 Years
SexALL
Healthy VolunteersNo
Study Sites51

Inclusion criteria: * Participants, who, in the opinion of the investigator, can and will comply with the requirements of the protocol. * Written or witnessed/thumb printed informed consent obtained from the participant prior to performance of any study-specific procedure. * A male or female betwee...

Countries:BelgiumBulgariaFranceGermanyHong KongItalyPhilippinesPolandRomaniaSingaporeSpainTaiwanThailandTurkey (Türkiye)United KingdomUnited StatesCanadaChinaJapanRussiaSouth AfricaSouth KoreaNetherlandsArgentinaMalaysia
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Frequently asked questions about GSK3228836

What is GSK3228836 used for?

GSK3228836 is an investigational small molecule being developed for the treatment of chronic hepatitis B. It is currently in Phase 2 clinical development and has been studied in multiple trials enrolling over 600 participants with chronic hepatitis B.

Who makes GSK3228836?

GSK3228836 is being developed by GSK plc, a global biopharmaceutical company. GSK plc trades on the London Stock Exchange and as an American Depositary Receipt under the ticker GSK on the New York Stock Exchange.

What phase is GSK3228836 in?

GSK3228836 is in Phase 2 clinical development for chronic hepatitis B. It is an investigational drug, meaning it has not been approved by regulatory authorities. Four clinical trials have been completed, including Phase 1 and Phase 2 studies.

What clinical trials has GSK3228836 been in?

GSK3228836 has been studied in four completed clinical trials. The largest, NCT04449029, enrolled 457 participants with chronic hepatitis B. Other trials include NCT04544956, a mechanistic study with 12 participants, NCT04676724, a sequential treatment study with 108 participants, and NCT04971928, a pharmacokinetic study in 24 participants.

Is GSK3228836 the same as bepirovirsen?

GSK3228836 is also known as bepirovirsen. It is an investigational antisense oligonucleotide being developed by GSK plc for the treatment of chronic hepatitis B. The drug has been evaluated in Phase 2 clinical trials, including studies combining it with peginterferon.