ProQR Announces Year End 2024 Operating and Financial Results Axiomer ADAR-mediated RNA editing pipeline advancing across liver and CNS programs, with CTA filing on track for Q2 2025 for lead program AX-0810 targeting NT

Year End 2024 Operating and Financial Results

LEIDEN, Netherlands &

CAMBRIDGE, Mass., March 13, 2025 - ProQR Therapeutics NV. (Nasdaq: PRQR) (ProQR), a company dedicated to changing lives through

transformative RNA therapies based on its proprietary Axiomer RNA editing technology platform, today reported its financial and operating

results for the year ended December 31, 2024, and provided a business update.

to advance our Axiomer ADAR-mediated RNA editing platform and pipeline, ProQR is entering a pivotal phase of growth and innovation,"

said Daniel A. de Boer, Founder and Chief Executive Officer of ProQR. "With up to four clinical trial data readouts anticipated

in 2025 and 2026, including topline data for our NTCP program later this year, we believe we are poised to demonstrate the broad therapeutic

potential of RNA editing. Our growing discovery pipeline, now including CNS applications, leading intellectual property portfolio, and

strategic partnerships - such as our $3.9 billion collaboration with Eli Lilly and partnership with the Rett Syndrome Research

Trust - reinforce our leadership in the field."

De Boer continued, "Our

successful financing in October 2024 further strengthens our financial foundation, providing cash runway into mid-2027. With this

strong position, we believe we are well equipped to advance key upcoming milestones, including submission of the CTA for our first RNA

editing pipeline program, AX-0810, on track for Q2 2025 and there following our first clinical data readout in Q4 of this year, as well

as continued progress across our pipeline and partnered programs. We remain committed to driving forward the next era of transformative

RNA editing therapeutics for patients in need."

Anticipated Upcoming Events

Year End 2024 Financial Highlights

2024, ProQR held cash and cash equivalents of 149.4 million, compared to 118.9 million at December 31, 2023.

Net cash used in operating activities during the full year ended December 31, 2024 was 36.4 million, compared to

21.5 million net cash generated by operating activities for the same period in 2023. The Company experienced a net positive cash

flow from operating activities in 2023 primarily due to the receipt of the Lilly up-front payment of $60 million in February 2023.

Research and development

costs for the year ended December 31, 2024 were 36.4 million, compared to 25.1 million for the same period

in 2023. The increase in research and development costs for the year ended December 31, 2024 compared to 2023 was due primarily

to increased investments in the platform and advancing pipeline programs.

General and administrative

costs for the year ended December 31, 2024 were 13.7 million, compared to 16.2 million for the same period in

Net loss for the year

ended December 31, 2024 was 27.8 million or 0.32 per diluted share, compared to 27.7 million, or

0.35 per diluted share for the year ended December 31, 2023.

For further financial information for

the period ended December 31, 2024, please refer to our 2024 Annual Report on Form 20-F and our Statutory Annual Report,

which will be available on our website, www.proqr.com under Financials and Filings.

ProQR is pioneering a next-generation

RNA base editing technology called Axiomer , which could potentially yield a new class of medicines for diverse types

of diseases. Axiomer "Editing Oligonucleotides", or EONs, mediate single nucleotide changes to RNA in a

highly specific and targeted way using molecular machinery that is present in human cells called ADAR (Adenosine Deaminase Acting on

RNA). Axiomer EONs are designed to recruit and direct endogenously expressed ADARs to change an Adenosine (A) to

an Inosine (I) in the RNA - an Inosine is translated as a Guanosine (G) - correcting an RNA with a disease-causing

mutation back to a normal (wild type) RNA, modulating protein expression, or altering a protein so that it will have a new function that

helps prevent or treat disease.

ProQR Therapeutics is dedicated to changing

lives through the creation of transformative RNA therapies. ProQR is pioneering a next-generation RNA technology called Axiomer ,

which uses a cell's own editing machinery called ADAR to make specific single nucleotide edits in RNA to reverse a mutation or

modulate protein expression and could potentially yield a new class of medicines for both rare and prevalent diseases with unmet need.

Based on our unique proprietary RNA repair platform technologies we are growing our pipeline with patients and loved ones in mind.

more about ProQR at www.proqr.com.

Forward Looking Statements

This press release contains forward-looking

statements. All statements other than statements of historical fact are forward-looking statements, which are often indicated by terms

such as "continue," "anticipate," "believe," "could," "estimate," "expect,"

"goal," "intend," "look forward to", "may," "plan," "potential," "predict,"

"project," "should," "will," "would" and similar expressions. Such forward-looking statements

include, but are not limited to, statements regarding our business, technology, strategy, preclinical and clinical model data, our initial

pipeline targets and the upcoming strategic priorities and milestones related thereto, the continued advancement of our lead development

pipeline programs, including ongoing and planned clinical trials, the anticipated timing of initial

clinical data readouts across multiple programs in 2025 and 2026, and the filing of a CTA application for our lead

program, AX-0810, in Q2 2025, our Axiomer platform, including the continued development and advancement of our

Axiomer platform, the therapeutic potential of our Axiomer RNA editing oligonucleotides and product candidates, the timing, progress

and results of our preclinical studies and other development activities, including the release of data related thereto, our patent estate,

including our anticipated strength and our continued investment in it, as well as the timing of our clinical development, the potential

of our technologies and product candidates, the collaboration with Lilly and the intended benefits thereof, including potential milestones,

exercise of an option to expand targets and the receipt of an opt-in payment, our ability to selectively form new partnerships and enter

into future collaborations, and our financial position and cash-runway. Forward-looking statements are based on management's beliefs

and assumptions and on information available to management only as of the date of this press release. Our actual results could differ

materially from those expressed or implied by these forward-looking statements for many reasons, including, without limitation, the risks,

uncertainties and other factors in our filings made with the Securities and Exchange Commission, including certain sections of our annual

report filed on Form 20-F. These risks and uncertainties include, among others, the cost, timing and results of preclinical studies

and clinical trials and other development activities by us and our collaborative partners whose operations and activities may be slowed

or halted shortage and pressure on supply and logistics on the global market, economic sanctions and international tariffs; the likelihood

of our preclinical and clinical programs being initiated and executed on timelines provided and reliance on our contract research organizations

and predictability of timely enrollment of subjects and patients to advance our clinical trials and maintain their own operations; our

reliance on contract manufacturers to supply materials for research and development and the risk of supply interruption from a contract

manufacturer; the potential for future data to alter initial and preliminary results of early-stage clinical

trials; the unpredictability of the duration and results of the regulatory review of applications or clearances that are necessary to

initiate and continue to advance and progress our clinical programs; the ability to secure, maintain and realize the intended benefits

of collaborations with partners, including the collaboration with Lilly; the possible impairment of, inability to obtain, and costs to

obtain intellectual property rights; possible safety or efficacy concerns that could emerge as new data are generated in research and

development; general business, operational, financial and accounting risks, and risks related to litigation and disputes with third parties;

and risks related to macroeconomic conditions and market volatility resulting from global economic developments, geopolitical instability

and conflicts. Given these risks, uncertainties and other factors, you should not place undue reliance on these forward-looking statements,

and we assume no obligation to update these forward-looking statements, even if new information becomes available in the future, except

ProQR Therapeutics N.V.