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Immutep s Efti with Radiotherapy & KEYTRUDA (pembrolizumab) Meets Primary Endpoint in Phase II for Soft Tissue Sarcoma Novel combination with efti has met the trial s primary endpoint of tumour hyalinization/fibrosis in

Key Takeaway: Immutep Limited's Efti, when combined with radiotherapy and KEYTRUDA (pembrolizumab), has met its primary endpoint in the EFTISARC-NEO Phase II trial for soft tissue sarcoma, achieving a tumour hyalinization/fibrosis rate significantly above historical data. The trial's success indicates the potential for improved survival outcomes in patients with this aggressive cancer type. Results will be presented at a future medical meeting, highlighting the importance of this research within the oncology field.

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POSITIVE FACTORS

  • Efti significantly exceeded the study's prespecified median of 35% tumour hyalinization/fibrosis.
  • The combination therapy demonstrated promising efficacy with a median of 50% tumour hyalinization/fibrosis in preliminary analyses.
  • The study addresses a high unmet need in treating soft tissue sarcoma, a rare and aggressive cancer type.

Full Press Release Details

Immutep s Efti with Radiotherapy &
KEYTRUDA (pembrolizumab) Meets
Primary Endpoint in Phase II for Soft
SYDNEY, AUSTRALIA May 26, 2025 Immutep Limited (ASX: IMM; NASDAQ: IMMP) ( Immutep or
the Company ), a late-stage immunotherapy company targeting cancer and autoimmune diseases, today announces the investigator-initiated EFTISARC-NEO Phase II trial evaluating eftilagimod alfa (efti)
with radiotherapy plus KEYTRUDA (pembrolizumab) in the neoadjuvant setting for resectable soft tissue sarcoma (STS) has met its primary endpoint. The novel combination significantly exceeded
the study s prespecified median of 35% tumour hyalinization/fibrosis versus 15% for historical data from radiotherapy alone in patients with resectable soft tissue sarcoma (STS).
Tumour hyalinization/fibrosis is an early surrogate endpoint at the time of surgical resection that has been associated with improved overall survival and
recurrence-free survival for STS patients.1,2 The trial s investigators at the Maria Sk odowska-Curie National Research Institute of Oncology (MSCNRIO) in Warsaw, the national reference
centre for STS in Poland, plan to present detailed results from the study at a future medical meeting.
Katarzyna Kozak, M.D., Ph.D., and
Pawe Sobczuk, M.D., Ph.D., medical oncologists at the Department of Soft Tissue/Bone Sarcoma and Melanoma at MSCNRIO and the trial s principal investigators, said: It is very encouraging to see the
chemotherapy-free combination with efti far exceed the ambitious target we initially set for the trial s primary endpoint in resectable soft tissue sarcoma. These results support our belief that efti s activation of antigen-presenting
cells, and in turn a broad adaptive and innate immune response, helps transform the immunosuppressed tumour microenvironment of soft tissue sarcomas leading to strong anti-cancer efficacy. There remains a very high unmet need in this aggressive
orphan cancer indication and we look forward to presenting detailed results at a medical meeting later this year.
As previously announced at the
Connective Tissue Oncology Society (CTOS) Annual Meeting in November 2024, the combination therapy demonstrated significant efficacy with a median of 50% tumour hyalinization/fibrosis in a preliminary analysis of 21 patients with resectable STS
available for primary endpoint assessment. The EFTISARC-NEO study, which is primarily funded with a grant from the Polish government awarded by the Polish Medical Research Agency program, subsequently
completed enrolment of 40 patients in January 2025.
STS is an orphan disease with high unmet medical need and a poor prognosis for patients. The
incidence of STS varies in different regions globally. In the United States, the number of new STS cases in 2025 is estimated to be ~13,520 with ~5,420 deaths, according to the American Cancer
For more information on EFTISARC-NEO, visit
clinicaltrials.gov (NCT06128863).
About Eftilagimod Alfa (efti)
Efti is Immutep s proprietary soluble LAG-3 protein and MHC Class II agonist that stimulates both innate and
adaptive immunity for the treatment of cancer. As a first-in-class antigen presenting cell (APC) activator, efti binds to MHC (major histocompatibility complex)
Class II molecules on APC leading to activation and proliferation of CD8+ cytotoxic T cells, CD4+ helper T cells, dendritic cells, NK cells, and monocytes. It also upregulates the expression of key biological molecules like IFN- and CXCL10 that further boost the immune system s ability to fight cancer.
Efti is under evaluation for a variety of solid
tumours including non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), and metastatic breast cancer. Its favourable safety profile enables various combinations, including with anti-PD-[L]1 immunotherapy and/or chemotherapy. Efti has received Fast Track designation in first line HNSCC and in first line NSCLC from the United States Food and Drug
Administration (FDA).
late-stage biotechnology company developing novel immunotherapies for cancer and autoimmune disease. The Company is a pioneer in the understanding and advancement of therapeutics related to Lymphocyte Activation
Gene-3 (LAG-3), and its diversified product portfolio harnesses LAG-3 s ability to stimulate or suppress the immune
response. Immutep is dedicated to leveraging its expertise to bring innovative treatment options to patients in need and to maximise value for shareholders. For more information, please visit www.immutep.com.
1. Schaefer IM et al. Histologic Appearance After Preoperative Radiation Therapy for Soft Tissue Sarcoma: Assessment of the European Organization for
Research and Treatment of Cancer-Soft Tissue and Bone Sarcoma Group Response Score. Int J Radiat Oncol Biol Phys. 2017 Jun 1;98(2):375-383. doi: 10.1016/j.ijrobp.2017.02.087. Epub 2017 Feb 24. PMID: 28463157.
2. Rao SR et al. Extent of tumor fibrosis/hyalinization and infarction following neoadjuvant radiation therapy is associated with improved survival
in patients with soft-tissue sarcoma. Cancer Med. 2022 Jan;11(1):194-206. doi: 10.1002/cam4.4428. Epub 2021 Nov 27. PMID: 34837341; PMCID: PMC8704179.
3. American Cancer Society statistics:
KEYTRUDA is a registered trademark of Merck Sharp & Dohme LLC, a subsidiary of Merck & Co., Inc., Rahway, NJ, USA.
Australian Investors/Media:
+61 (0)406 759 268; catherine.strong@sodali.com
Chris Basta, VP, Investor Relations and
Corporate Communications
+1 (631) 318 4000; chris.basta@immutep.com
This announcement was authorised for release by the CEO of Immutep Limited.
Immutep Limited, Level 32, Australia Square, 264 George Street, Sydney NSW 2000, Australia

Frequently Asked Questions

What is the primary focus of the EFTISARC-NEO trial?

The trial evaluates eftilagimod alfa with radiotherapy and KEYTRUDA for soft tissue sarcoma.

What was the primary endpoint achieved in this trial?

The trial achieved a 35% tumor hyalinization/fibrosis, exceeding the historical 15%.

Who conducted the EFTISARC-NEO trial?

The trial was conducted by investigators at the MSCNRIO in Warsaw, Poland.

What potential does eftilagimod alfa have?

Efti stimulates immune responses, enhancing the treatment of various solid tumors.

What was the patient enrollment for the trial?

The EFTISARC-NEO trial enrolled 40 patients by January 2025.

Last updated: May 26, 2025