Disclaimer All statements other than statements of historical facts included in this presentation that address activities, events or developments that we expect, believe or anticipate will or may occur in the future are

Investor Presentation MAY 2026 Exhibit

Disclaimer All statements other than

statements of historical facts included in this presentation that address activities, events or developments that we expect, believe or anticipate will or may occur in the future are forward-looking statements. These statements are based on

management's current expectations, assumptions, estimates and beliefs. Although we believe that we have a reasonable basis for forward-looking statements contained herein, we caution you that they are based on current expectations about future

events affecting us and are subject to risks, uncertainties and factors relating to our operations and business environment, all of which are difficult to predict and many of which are beyond our control, that may cause our actual results to differ

materially from those expressed or implied by forward-looking statements in this presentation. These potential risks and uncertainties that could cause actual results to differ materially from those described in forward-looking statements include,

without limitation, our ability to successfully commercialize our iDose TR and Epioxa therapies; the impact of general macroeconomic conditions including foreign currency fluctuations and future public health crises; supply and/or manufacturing

disruptions, including those impacting our principal revenue-producing products, including the risk of recalls or serious safety issues with our products; our ability to achieve or sustain profitability, generate sales of our commercialized products

and develop and commercialize additional products; risks associated with our international operations; our ability to meet our customers' expectations for the quality or delivery of our products; the potential for misuse of our products; our

ability to manage our growth and meet customer demand; the success of our acquisitions, collaborations, in licensing agreements, joint ventures, alliances or partnerships with third parties; our ability to protect our information systems against

cyber threats and cybersecurity incidents, and to comply with state, federal and foreign data privacy laws and regulations; risks related to the implementation of artificial intelligence and machine learning technologies; the availability of net

operating loss tax carryforwards; risks related to our capped call transactions; changes to domestic or foreign healthcare laws or trade policies, which could impact our profitability; the high cost of regulatory compliance, including the

requirements of participation in federal healthcare programs such as Medicare and Medicaid and regulations for the approval and sale and marketing of our products and of our manufacturing processes; risks related to securing or maintaining adequate

coverage or reimbursement by government or third-party payors the lengthy and expensive clinical trial process and the uncertainty of timing and outcomes from any particular clinical trial or regulatory approval processes; and our ability to

protect, and the expense and time-consuming nature of protecting, our intellectual property against third parties and competitors and the impact of any claims against us for infringement or misappropriation of third party intellectual property

rights and any related litigation. These and other known risks, uncertainties and factors are described in detail under the caption "Risk Factors" and elsewhere in our filings with the Securities and Exchange Commission (SEC), including

our Annual Report on Form 10-K for the year ended December 31, 2025, which was filed with the SEC on February 23, 2026, and our Quarterly Report on Form 10-Q for the year ended March 31, 2026, which was filed with the SEC on April 30, 2026. Our

filings with the SEC are available in the Investor Section of our website at www.glaukos.com or at www.sec.gov. In addition, information about the risks and benefits of our products is available on our website at www.glaukos.com. All forward-looking

statements included in this presentation are expressly qualified in their entirety by the foregoing cautionary statements. You are cautioned not to place undue reliance on the forward-looking statements in this presentation, which speak only as of

the date hereof. We do not undertake any obligation to update, amend or clarify these forward-looking statements whether as a result of new information, future events or otherwise, except as may be required under applicable securities law.

WE'LL Innovation is at the core

of everything we do. We push the limits of science and technology to solve unmet needs in chronic eye diseases for the benefit of patients worldwide. Dropless Therapies Challenging conventional paradigms to advance the standards of care and improve

outcomes Proven New Market Pioneer Building durable new markets to better serve physicians and patients Solutions Oriented; Creating & Exploiting Newest Technologies Unique hybrid pharmaceutical-device company transforming treatment options and

quality of life for large and/or underserved patient populations GO FIRST

Best-in-Class Growth Company with

Industry-Leading Pipeline 13 Disclosed pipeline programs 12 Currently commercialized products iStent Micro-Scale iDose Sustained-Release iLink Bio-Activated iLution Eyelid Delivery Retina XR Bio-Erodible IVT Ocular Hypertension and Open-Angle

Glaucoma Keratoconus Blepharitis Myopia AMD DME RVO 30 % 2025 topline growth 20 % 10-yr revenue CAGR 800 $ Million Invested in R&D since 2018 + + +

Two Unmet Clinical Needs = Robust

Long-Term Growth Opportunities Fundamentally resets how keratoconus is diagnosed, managed and treated to dramatically improve patient awareness and access to effective care INTERVENTIONAL KERATOCONUS Radically improves the conventional "drops

first" care paradigm with standalone therapies shown to slow disease progression and reduce drug burden INTERVENTIONAL GLAUCOMA

Major Problem in Glaucoma Care:

Non-Compliance with Topical Eye Drops 1 Nordstrom BL et al. Am J Ophthalmol. 2005;140(4): 598-606. % 90 are non-compliant with topical drops 50 ~ % purposely discontinue topical drops within 6 months Patients aren't compliant with drop

regimens1 > Topical medications used as a supplement ("bridge therapy") as needed LEGACY GLAUCOMA PARADIGM Reactive Drops first and always Compliance dependent TOPICAL DROPS MORE DROPS SLT (AND DROPS) FILTRATION SURGERY MORE

FILTRATION SURGERY Drops as supplement ("bridge therapy") if needed NEW INTERVENTIONAL GLAUCOMA (IG) PARADIGM SLT PROCEDURAL PHARMACEUTICALS MIGS MIBS FILTRATION SURGERY Procedural interventions empower the HCP to actively manage

glaucoma care, preserve vision and improve quality of life Proactive Personalized Compliance independent

Glaukos is Ideally Positioned to Lead

the IG Paradigm Shift OCULAR HYPERTENSION ADVANCED MODERATE MILD REFRACTORY The IG workhorse: Foundational therapy across the entire disease stage spectrum For patients who have failed surgical and medical therapy IG PARADIGM SLT PROCEDURAL

PHARMACEUTICALS MIGS MIBS FILTRATION SURGERY Topical meds as bridge therapy Combo-cataract procedures Strong real-world clinical outcomes Highly favorable HCP feedback Significant growth in the number of cases and trained surgeons Positive iDose TR

iDose TR: Long-Duration Drug Therapy

Addresses Patient Non-Compliance Anchors securely into scleral tissue for continual elution of unique, highly concentrated travoprost directly into anterior chamber Designed to deliver up to 3 years of drug therapy; proven favorable safety and

tolerability profile First and only glaucoma procedural pharmaceutical FDA-approved for re-administration (Jan 2026) IG PARADIGM SLT PROCEDURAL PHARMACEUTICALS MIGS MIBS FILTRATION SURGERY Topical meds as bridge therapy Excellent clinical results

fuel HCP adoption vs 2,190 Drops over 3 years4 81 of iDose TR subjects free of IOP-lowering topical meds at 12 months2 % 70 of iDose TR subjects well-controlled on same or fewer meds at 3 years3 % 1 iDose TR is indicated for the reduction of IOP in

patients with OAG or OHT 2 Singh, I Paul et al. Drugs vol. 84,10 (2024) 3 Data on file 4 Topical eye drop prescription regimen over 3-year period, BID 1 1.8mm x 0.5mm New Phase 3 data analysis demonstrates non-inferiority to topical timolol at 3

iDose TR is Just the Beginning IG

PARADIGM SLT PROCEDURAL PHARMACEUTICALS MIGS MIBS FILTRATION SURGERY Topical meds as bridge therapy Designed to streamline insertion to help facilitate potential future in-office administration IND amendment filed December 2025; completed patient

enrollment in Phase 3b study Initial human factors study indicates strong user preference (~90% favorability)1 Designed to carry ~2x the drug capacity vs current iDose TR, supporting potentially longer duration Phase 2a initial results indicate

robust IOP-lowering1 Advancing Phase 2b/3 clinical program Initial results of Phase 2a clinical trial show mean IOP reductions of 8.6-10.8mmHg through Month 31 Advancing 6+ future iDose platform products designed to expand iDose utilization and

applicability to support future growth, including: TRIO 1 Data on file iDose TRIO and iDose TREX are not approved by the FDA

iStent infinite: Best-in-Class MIGS

Therapy for Long-Term IOP Control IG PARADIGM SLT PROCEDURAL PHARMACEUTICALS MIGS MIBS FILTRATION SURGERY Topical meds as bridge therapy 2012 Pioneered MIGS with iStent approval iStent technologies supported by 300+ peer-reviewed publications 2018

Launched iStent inject 2022 2025 Launched iStent infinite for advanced glaucoma Completed enrollment in iStent infinite mild-to-moderate study iStent infinite backed by 14 YEARS of commercial MIGS leadership iStent infinite provides up to 240

of powerful outflow coverage Integrity Study Results Demonstrate Superior Safety & Efficacy Profile1 1 Ahmed, Iqbal Ike K et al. Ophthalmology and therapy vol. 14,5 (2025): 1005-1024

IG Represents the Next Wave for

Comprehensive Ophthalmology Practices 1 Market Scope 2025 Glaucoma Surgical Device Report 2 Market Scope 2024 Glaucoma Pharmaceutical Report; includes glaucoma and OHT patients; assumes bilateral rate of 1.8x IG creates "forever

patients" by providing comprehensive ophthalmic practices: Safe and effective glaucoma treatment options Ability to deliver proactive, continual care that may include multiple interventions over a patient's 20-year glaucoma treatment

journey IG PATIENT POPULATION2 22M eyes 13M eyes 12M eyes Prevalence Diagnosed Diagnosed & Treated annual us cataract procedures1 5M eyes Cataract surgery is: Typically a "one and done" procedure and a mainstay for most comprehensive

ophthalmic practices Undergoing significant pro fee reductions ~15-20% w/co-morbid glaucoma

Ophthalmic surgeons seek to offset

continued cataract surgery pro fee reductions, helping to drive adoption and utilization of IG therapy 1 3 In-office injectable delivery of iDose and repeat administration approval can drive inflection in iDose role and IG therapy adoption 2

Combination therapy has potential to become a preferred form of IG therapy 4 New sustained-release drug delivery products designed to offer longer duration have potential to fuel additional IG therapy adoption (iDose TREX) 5 PE groups are likely to

gravitate to IG therapy due to the significant clinical benefits and practice efficiency it provides 6 A new breed of IG specialist could emerge, conducting primarily injection therapy while vertically integrated ODs and OD referral networks provide

preparatory patient services IG: A Vision for the Next 10 Years By 2035, US ophthalmologists could be performing as many IG procedures as cataract procedures

Product Roadmap Underscores

Extensive IG Product Depth PRODUCT PATIENT STATUS iStent / iStent inject / iStent inject W Mild-to-Moderate Glaucoma with Cataract FDA Approved (2012, 2018, 2020) iStent infinite Glaucoma (failed on prior therapy) FDA Cleared (2022) iStent infinite

Glaucoma (label expansion) Active PMA Study / EU MDR Cert (2025) PRESERFLO MicroShunt Advanced-Refractory Glaucoma OUS approved / US Active IDE Study iDose TR Ocular Hypertension - Glaucoma FDA Approved (2023) iDose TRIO Ocular Hypertension -

Glaucoma Phase 3b iDose TREX Ocular Hypertension - Glaucoma Phase 2b/3 iDose Next Generation Ocular Hypertension - Glaucoma Pre-Clinical Radius XR Wearable Patient Engagement & Diagnostic System FDA Cleared iAccess Precision Goniotomy FDA

Cleared Mitosol Adjunct to Glaucoma Filtration Surgery FDA Approved

Two Unmet Clinical Needs = Robust

Long-Term Growth Opportunities Fundamentally resets how keratoconus is diagnosed, managed and treated to dramatically improve patient awareness and access to effective care INTERVENTIONAL KERATOCONUS INTERVENTIONAL GLAUCOMA Radically improves the

conventional "drops first" care paradigm with standalone therapies shown to slow disease progression and reduce drug burden

Keratoconus (KC): Debilitating,

Sight-Threatening Rare Disease Topical medications used as a supplement ("bridge therapy") as needed OR KC is characterized by thinning and steepening of the cornea Typical onset in teenage years, most aggressively advancing in patients

under age 30 ~90% of cases are bilaterial Can lead to loss of visual function and blindness if left untreated ~20% of untreated patients ultimately require a corneal transplant, making KC a leading cause of penetrating keratoplasty in the US Since

2016 approval, Photrexa has been the only FDA-approved corneal cross-linking (CXL) therapy shown to slow or halt progression 300+ peer-reviewed publications support its performance and safety. However, newly diagnosed progressive KC eyes are getting

access to Photrexa today1 1 in 5 WHY? < 1 Company analysis using annual incidence based on the disease and population demographics

KC: Vastly Underdiagnosed,

Misdiagnosed, Undertreated Disease Topical medications used as a supplement ("bridge therapy") as needed 1 Kreps EO, et al. Cont Lens Anterior Eye. 2021:44(3):101 2 Based on annual Glaukos procedure volumes and internal analysis 3 Syed

ZA, et al. Ophthalmology. 2024;131(8):892-901. optimizing patient access requires a new standard of care and a coordinated, patient-centric approach essential for this rare disease Onerous, fragmented journey means only a fraction of patients

ultimately receive sight-saving therapy Poor Awareness Levels Managing Symptoms vs Disease Pain & Recovery Time w/ Epi-off Procedure Major Payer Hurdles 40 of proven KC patients decline or significantly delay therapy2 > % 70 % of these

KC patients had reached TKC Stage 2 (moderate severity KC) or higher by the time of diagnosis1 Retrospective cohort study of newly diagnosed KC patients showed that 12 % underwent CXL treatment3 18 13 of KC patients are diagnosed before age %

Transformative Epioxa Therapy

Ushers in New Standard of KC Care First and only FDA-approved epithelium-on treatment Incision-free, topical drug therapy catalyzed by oxygen and light Designed to slow or halt KC progression with a single administration Streamlined Procedure and

Faster Recovery Time More Desirable Patient Experience Now Commercially Available (March 2026) Supplemental oxygen essential for efficiency

Rare Disease: Significant

Investment to Dramatically Improve KC Patient Access Patient Education Advocacy Engagement Provider Awareness, Detection Centers & Tools Patient Service & Market Access Support Integrated IT Deployment Epioxa Sites of Care Specialty Pharma

Support Engaged OD-MD Networks OTHER OPHTHALMIC RARE DISEASE PHARMACEUTICALS

Launch Timing for Epioxa & New

Rare Disease Infrastructure 1Q 26 2Q 26 3Q 26 4Q 26 2027+ Go live with patient service and support hub Drug availability/launch Establish site of care network Establish payer coverage Support advocacy efforts and contracting processes J-Code

Effective Date Accelerate marketing spend to support increased patient awareness and education Expand body of clinical evidence with peer-reviewed publications

WHAT IF? CUSTOMIZED, SPHERICAL

THERAPY Use biomechanical modeling to deliver customized, patterned treatment that matches each patient's unique corneal topography Use proprietary algorithm to precisely target UV energy for maximum CXL efficacy 3rd Gen iLink is not approved

by the FDA Next Game-Changing Program: Customized, Spherical Therapy for KC Currently in Phase 2 clinical trials Built on Epioxa advantages while further streamlining and enhancing patient experience Potential to be another game-changing advancement

in KC care DESIGNED TO:

Product Roadmap for Sustained

Leadership in Effective KC Care PRODUCT PATIENT STATUS Photrexa (Epi-off) Keratoconus FDA Approved (2016) Epioxa (Epi-on) Keratoconus FDA Approved (2025) iLink 3rd Generation Keratoconus Phase 2 iVeena (IVMED-80) Keratoconus Phase 2 iLinko2n KC

Screening Device Keratoconus Pre-Submission

Novel Eyelid Drug Delivery Platform

Spearheads Glaukos' Entry into Anterior Segment Part D Pharmaceuticals 1 O'Dell L, Dierker DS, Devries DK, et al. Psychosocial Impact of Demodex Blepharitis. Clin Ophthalmol. 2022;16:2979-2987 2 Publicly available market estimates iLution

therapies are not approved by the FDA Transdermal cream applied to eyelid offers potential benefits vs prescription eye drops Primarily focused on solutions for multiple chronic eye diseases and disorders Easier administration Faster onset of action

Fewer side effects Better compliance PLATFORM FOCUS Demodex Bletharitis Progressive Myopia Demodex blepharitis program in Phase 2 clinical stage with patient enrollment advancing Eyelid delivery of potent transdermal physostigmine cream designed to