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TP-03, 0.25%

Phase 3

Blepharitis | Small molecule | Ophthalmology |Tarsus Pharmaceuticals, Inc.|Last Updated: Oct 1, 2025

Target and mechanism

ModalitySmall molecule

Also known as TP-03 (Lotilaner Ophthalmic Solution), 0.25%, TP-03

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials2
Total Enrollment833

FDA Designations

No designations recorded

Clinical trial landscape

TP-03, 0.25% · 4 trials · 4 indications

Phase 3 1Phase 2 2Phase 1 1
NCT04784091Trial to Evaluate the Safety and Efficacy of TP-03 for the Treatment of Demodex BlepharitisBlepharitis
COMPLETED412 Analytics
PHASE3COMPLETED
Trial to Evaluate the Safety and Efficacy of TP-03 for the Treatment of Demodex Blepharitis
BlepharitisUnlock trial analytics

Study Endpoints

Primary Endpoints

The Proportion of Participants Cured Based on Their Collarette Score of 0 for the Upper Eyelid.
43 days

The proportion of subjects cured where cure is defined as 0-2 lashes with collarettes on the upper eyelid of the analysis eye. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values

Number of Participants With Treatment Emergent Adverse Events (TEAEs)
85 days

Number of participants with TEAEs related (definitely or potentially) to treatment summarized by MedDRA preferred term.

Proportion of Participants Cured Based on a Collarette Score at Day 43.
43 days

The proportion of participants cured where cure is defined as 0-2 lashes with collarettes on the upper eyelid of the analysis eye. The primary outcome analysis is the combined result from 20 analyses each with missing data imputed. Missing data were imputed per the method described in the SAP. The least square mean is computed from the result of the 20 analyses with imputed missing values.

To evaluate the concentration of lotilaner in blood multiple doses of TP-03, 0.25% in whole blood following topical ocular administration in healthy adult subjects for 42 days.
42 Days

The primary PK endpoints following single and multiple dose administration will include whole blood PK parameters for lotilaner Cmax at various times

Incidence of treatment emergent adverse events (TEAEs)
42 Days

Safety will be evaluated through the incidence rate of TEAEs

Clinically significant changes from Baseline chemistry laboratory tests
42 Days

Evaluate the safety of TP-03 through clinically significant changes from Baseline chemistry laboratory tests

Clinically significant changes from Baseline hematology laboratory tests
42 Days

Evaluate the safety of TP-03 through clinically significant changes from Baseline hematology laboratory tests

Clinically significant changes from Baseline physical examinations
42 Days

Safety will be evaluated through review of clinically significant changes in physical examinations from Baseline

Clinically significant changes from Baseline electrocardiograms (ECGs)
42 Days

Safety will be evaluated through review of clinically significant changes in electrocardiograms from Baseline

Clinically significant changes from Baseline vitals
42 Days

Safety will be evaluated through review of clinically significant changes from Baseline vital signs (including temperature \[degrees Celsius\], pulse rate \[beats per minute\], respiration rate \[breaths per minute\], and changes in systolic and diastolic blood pressure \[mmHg\]) from Baseline

Clinically significant changes from Baseline corrected distance visual acuity
42 Days

Safety will be evaluated through review of clinically significant changes in corrected distance visual acuity from Baseline

Clinically significant changes from Baseline non-mydriatic fundus photographs
42 Days

Safety will be evaluated through review of clinically significant changes in non-mydriatic fundus photographs from Baseline

Clinically significant changes from Baseline intraocular pressure (IOP) measurement
42 Days

Safety will be evaluated through review of clinically significant changes in IOP from Baseline

Secondary Endpoints

The Proportion of Participants With Their Demodex Mites Eradicated.
43 days
The Proportion of Participants Cured Based on a Composite of Collarette Score and Erythema Score
43 days
The Proportion of Participants Cured Based on Erythema Score.
43 days
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Study Design & Arms

AllocationRANDOMIZED
MaskingQUADRUPLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
ActiveEXPERIMENTALTP-03, lotilaner ophthalmic solution, 0.25%, administered topically twice a day for approximately 43 days
ControlPLACEBO_COMPARATORVehicle of TP-03 ophthalmic solution, administered topically twice a day for approximately 43 days
BID DosingEXPERIMENTALTP-03, lotilaner ophthalmic solution, 0.25% administered topically twice a day and TP-03 vehicle administered once a day to maintain masking for approximately 85 days
TID DosingEXPERIMENTALTP-03, lotilaner ophthalmic solution, 0.25% administered topically three times a day for approximately 85 days
TP-03 (Lotilaner Ophthalmic Solution), 0.25%EXPERIMENTALTP-03, topical ocular administration in healthy adults. Single and multiple doses for 42 days.

Interventions

NameTypeDescription
TP-03DRUGTP-03, lotilaner ophthalmic solution, 0.25%, administered twice a day
TP-03 VehicleDRUGVehicle of TP-03 ophthalmic solution, administered twice a day
TP-03, 0.25%DRUGTP-03, lotilaner ophthalmic solution, 0.25%, administered twice a day
TP-03 (Lotilaner Ophthalmic Solution), 0.25%DRUGA single drop of the ophthalmic solution will be instilled in each eye on the morning of Day 1 and then twice a day (in the morning and in the evening, approximately 12 hours apart) starting on Day 2 for 40 consecutive days (Days 2 to 41). Thereafter, a single drop of the ophthalmic solution will be instilled in each eye on the morning of Day 42, for a total of 82 consecutive doses administered in each eye.
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Eligibility Criteria

Age Range18 Years to N/A
SexALL
Healthy VolunteersNo
Study Sites21

Inclusion Criteria: * Be willing to sign the informed consent and deemed capable of complying with the requirements of the study protocol * Meet all of the following criteria in at least one eye: Have more than 10 lashes with collarettes present on the upper lid; have at least mild erythema of the ...

Countries:United StatesCanada
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Competitive Landscape -Blepharitis 2 trials

Top 1 of 2 competitors

CompanyTickerTrialsLead PhaseDrugs
Glaukos CorpGKOS1PHASE4GLK-321
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Frequently asked questions about TP-03, 0.25%

What is TP-03, 0.25%?

TP-03, 0.25% is an investigational small molecule ophthalmic solution developed by Tarsus Pharmaceuticals for ophthalmic conditions including Demodex blepharitis and Meibomian Gland Dysfunction. It is a topical eye drop formulation studied in randomized, double-blind, placebo-controlled clinical trials. It has completed Phase 3 development and is not yet approved.

What is TP-03, 0.25% used for?

TP-03, 0.25% is being studied for the treatment of blepharitis, including Demodex blepharitis, and Meibomian Gland Dysfunction. Clinical trials have also evaluated it in healthy subjects for pharmacokinetic purposes. These are investigational uses, and the drug has not been approved for any indication.

Who makes TP-03, 0.25%?

TP-03, 0.25% is developed by Tarsus Pharmaceuticals, Inc., which trades on the Nasdaq under the ticker TARS. The company is the sponsor of the clinical trials evaluating the ophthalmic solution for blepharitis and Meibomian Gland Dysfunction.

What phase is TP-03, 0.25% in?

TP-03, 0.25% has completed Phase 3 clinical development. A Phase 3 trial evaluated the safety and efficacy of TP-03 for the treatment of Demodex blepharitis. The drug is investigational and has not been approved by the FDA for any indication.

What clinical trials is TP-03, 0.25% in?

TP-03, 0.25% has been studied in completed trials including NCT04784091, a Phase 3 trial in Demodex blepharitis with 412 participants, and NCT04475432, a Phase 2 trial in Demodex blepharitis with 421 participants. Additional completed studies include NCT05454956 in Meibomian Gland Dysfunction and NCT05138861, a Phase 1 pharmacokinetic study in healthy subjects.

Is TP-03, 0.25% the same as TP-03?

Yes. TP-03, 0.25% is also known as TP-03 (Lotilaner Ophthalmic Solution), 0.25%, or simply TP-03. These names refer to the same investigational ophthalmic solution developed by Tarsus Pharmaceuticals for blepharitis and Meibomian Gland Dysfunction.