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SPR720

Phase 2

Nontuberculous Mycobacterial Pulmonary Disease (NTM-PD) | Small molecule | Respiratory |Spero Therapeutics, Inc.|Last Updated: Oct 9, 2025

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials1
Total Enrollment25

FDA Designations

No designations recorded

Clinical trial landscape

SPR720 · 3 trials · 3 indications

Phase 2 1Phase 1 2
NCT05496374A Study to Evaluate the Efficacy, Safety, Tolerability, and Pharmacokinetics of SPR720 as Compared With Placebo for the Treatment of Participants With Mycobacterium Avium Complex (MAC) Pulmonary DiseaseNontuberculous Mycobacterial Pulmonary Disease (NTM-PD)
COMPLETED25 Analytics
PHASE2COMPLETED
A Study to Evaluate the Efficacy, Safety, Tolerability, and Pharmacokinetics of SPR720 as Compared With Placebo for the Treatment of Participants With Mycobacterium Avium Complex (MAC) Pulmonary Disease
Nontuberculous Mycobacterial Pulmonary Disease (NTM-PD)Unlock trial analytics

Study Endpoints

Primary Endpoints

Slope of the Weekly Sputum Log10 Colony Forming Units Per Millilitre (CFU/mL) Change From Day 1 Through 56 in micro-Intent to Treat (m-ITT) Population
Days 1 through 56 (end of the treatment [EOT])
Maximum Observed Plasma Concentration (Cmax) of Study Drug
Pre-dose and at multiple time points post-dose up to Day 8
Time to Attain Maximum Plasma Concentration (tmax) for Study Drug
Pre-dose and at multiple time points post-dose up to Day 8
Area Under the Concentration-time Curve From Time Zero to Dosing Interval (AUC0-τ) for Study Drug
Pre-dose and at multiple time points post-dose up to Day 8
Area Under the Concentration-time Curve from Time Zero to 24 Hours (AUC0-24) of SPR719 in plasma, ELF and AM
Predose on Day 6 and post-dose at multiple time points up to Day 8
Maximum Observed Concentration (Cmax) of SPR719 in plasma, ELF and AM
Predose on Day 6 and post-dose at multiple time points up to Day 8
Time to the Maximum Observed Concentration (Tmax) of SPR719 in plasma, ELF and AM
Predose on Day 6 and post-dose at multiple time points up to Day 8

Secondary Endpoints

Slope of the Weekly Sputum Log10 CFU/mL Change From Days 1 Through 28 in micro-ITT Population
Days 1 through 28
Slope of the Time to Positivity (TTP) using Mycobacteria Growth Indicator Tube (MGIT) on Samples of Induced Sputum From Days 1 Through 56 (EOT) in micro-ITT Population
Days 1 through 56 (EOT)
Change from Baseline in the Sputum Log10 CFU/mL in the micro-ITT Population
Days 1 through 56 (EOT)
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Study Design & Arms

AllocationRANDOMIZED
MaskingQUADRUPLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
Investigator Blind: PlaceboPLACEBO_COMPARATORParticipants received SPR720 matching-placebo capsules, orally, once daily (QD) for 56 days.
Investigator Blind: SPR720 500 mgEXPERIMENTALParticipants received SPR720 500 mg (250 mg × 2) capsules along with 2 SPR720 matching- placebo capsules, orally, QD for 56 days.
Investigator Blind: SPR720 1000 mgEXPERIMENTALParticipants received SPR720 1000 mg (250 mg × 4) capsules, orally, QD for 56 days.
Open-label: SPR720 1000 mgEXPERIMENTALParticipants received SPR720 1000 mg (250 mg × 4) capsules, orally, QD for 56 days.
Open-label: SPR720 500 mgEXPERIMENTALParticipants received SPR720 500 mg (250 mg × 2) capsules, orally, twice daily (BID) for 56 days.
SPR720 1000 mgEXPERIMENTALHealthy participants will receive SPR720 1000 milligrams (mg), orally, once daily (QD) for 7 days.
Azithromycin 500 mgEXPERIMENTALHealthy participants will receive azithromycin 500 mg, orally, QD for 7 days.
Ethambutol 800 mgEXPERIMENTALHealthy participants will receive ethambutol 800 mg, orally, QD for 7 days.
SPR720 1000 mg + Azithromycin 500 mg + Ethambutol 800 mgEXPERIMENTALHealthy participants will be co-administered SPR720 1000 mg, azithromycin 500 mg, and ethambutol 800 mg, orally, QD for 7 days.
Azithromycin 500 mg + Ethambutol 800 mgEXPERIMENTALHealthy participants will be co-administered azithromycin 500 mg, and ethambutol 800 mg, orally, QD for 7 days.
SPR720EXPERIMENTALHealthy participants will receive SPR720 1000 mg capsule, orally, for 7 days.

Interventions

NameTypeDescription
PlaceboDRUGPlacebo-matching capsules was administered orally.
SPR720 500 mgDRUGSPR720 500 mg (250 mg × 2 capsules) was administered orally.
SPR720 1000 mgDRUGSPR720 500 mg (250 mg × 4 capsules) was administered orally.
SPR720DRUGMultiple oral doses of SPR720 capsules.
AzithromycinDRUGMultiple oral doses of azithromycin.
EthambutolDRUGMultiple oral doses of ethambutol.
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Eligibility Criteria

Age Range18 Years to N/A
SexALL
Healthy VolunteersNo
Study Sites28

Inclusion Criteria: 1. Has a prior diagnosis of NTM-PD due to MAC according to American Thoracic Society (ATS) criteria 2. Has at least one prior lower respiratory culture (sputum or bronchoalveolar lavage \[BAL\]) positive for MAC in the 12 months prior to consent 3. Has an induced sputum culture ...

Countries:United States
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Frequently asked questions about SPR720

What is SPR720 used for?

SPR720 is an investigational small molecule being studied for the treatment of Nontuberculous Mycobacterial Pulmonary Disease (NTM-PD), specifically Mycobacterium Avium Complex (MAC) pulmonary disease. It has also been evaluated in healthy volunteers for pharmacokinetic studies. SPR720 is not approved and remains in clinical development.

Who makes SPR720?

SPR720 is being developed by Spero Therapeutics, Inc., a biopharmaceutical company traded on NASDAQ under the ticker SPRO. The company is conducting clinical trials to evaluate the drug's efficacy, safety, and pharmacokinetics in patients with NTM-PD and in healthy volunteers.

What phase is SPR720 in?

SPR720 has completed Phase 1 and Phase 2 clinical trials. The Phase 2 study evaluated its efficacy and safety in patients with MAC pulmonary disease, while Phase 1 studies assessed pharmacokinetics in healthy volunteers. SPR720 is investigational and has not received FDA approval.

What clinical trials is SPR720 in?

SPR720 has been studied in three completed trials: NCT05496374 (Phase 2, efficacy and safety in MAC pulmonary disease, 25 participants), NCT05955586 (Phase 1, intrapulmonary pharmacokinetics in healthy volunteers, 33 participants), and NCT05966688 (Phase 1, drug interaction study in healthy volunteers, 39 participants).

How does SPR720 work?

SPR720 is a small molecule that targets bacterial DNA gyrase, an enzyme essential for bacterial DNA replication. By inhibiting this target, SPR720 aims to treat infections caused by Mycobacterium Avium Complex. This mechanism is being evaluated in clinical trials for NTM-PD.