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ProQuad manufactured with recombinant Human Albumin

Phase 3

Measles | Monoclonal antibody | Infectious Disease |Merck & Company, Inc.|Last Updated: Jan 30, 2018

Target and mechanism

ModalityMonoclonal antibody

Also known as ProQuad® manufactured with recombinant Human Albumin (rHA)

Success Probability

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Market & Valuation

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Trial Design

RandomizedACTIVE_CONTROLLED
Total Trials1
Total Enrollment1,620

FDA Designations

No designations recorded

Clinical trial landscape

ProQuad manufactured with recombinant Human Albumin · 1 trial · 4 indications

Phase 3 1
NCT00566527Comparative Study of Immunogenicity and Safety of a 2-dose Regimen of ProQuad® Manufactured With rHA (V221-038)Measles
COMPLETED1,620 Analytics
PHASE3COMPLETED
Comparative Study of Immunogenicity and Safety of a 2-dose Regimen of ProQuad® Manufactured With rHA (V221-038)
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Study Endpoints

Primary Endpoints

Percentage of Participants in Arm 2 and Arm 3 Meeting Antibody Immunogenicity Response Criteria Following ProQuad® Dose 2
Day 132 (6 weeks after ProQuad® Dose 2)

Immunogenicity response rates were compared in participants with baseline seronegativity from Arm 2 (received ProQuad® Dose 1 at 11 months) and Arm 3 (received ProQuad® Dose 1 at 12 months). Measles, mumps and rubella antibody levels were determined using enzyme-linked immunosorbent assay (ELISA) and varicella antibody levels were determined with glycoprotein-based ELISA (gpELISA). Response and baseline seronegativity criteria were as follows: measles antibody titre ≥255 mIU/mL in participants with baseline titre \<255 mIU/mL; mumps antibody titre ≥10 ELISA Ab units/mL in participants with baseline titre \<10 ELISA Ab units mL; rubella antibody titre ≥10 IU/mL in participants with baseline titre \<10 IU/mL; varicella antibody titre ≥5 gpELISA units/mL in participants with baseline titre \<1.25 gpELISA units/mL.

Percentage of Participants in Arm 1 and Arm 3 Meeting Antibody Immunogenicity Response Criteria Following ProQuad® Dose 2
Day 132 (6 weeks after ProQuad® Dose 2)

Immunogenicity response rates were compared in participants with baseline seronegativity from Arm 1 (received ProQuad® Dose 1 at 9 months) and Arm 3 (received ProQuad® Dose 1 at 12 months). Measles, mumps and rubella antibody levels were determined using ELISA and varicella antibody levels were determined with gpELISA. Response and baseline seronegativity criteria were as follows: measles antibody titre ≥255 mIU/mL in participants with baseline titre \<255 mIU/mL; mumps antibody titre ≥10 ELISA Ab units/mL in participants with baseline titre \<10 ELISA Ab units mL; rubella antibody titre ≥10 IU/mL in participants with baseline titre \<10 IU/mL; varicella antibody titre ≥5 gpELISA units/mL in participants with baseline titre \<1.25 gpELISA units/mL.

Percentage of Participants With Solicited Injection-site Adverse Reactions
Day 1 to Day 4 (up to 4 days after ProQuad® Dose 1)

The solicited injection-site AEs erythema, swelling, and pain were monitored for 4 days after administration of ProQuad® Dose 1.

Percentage of Participants Experiencing Unsolicited Injection-site Adverse Reactions
Up to Day 28 (up to 28 days after ProQuad® Dose 1)

The percentage of participants with unsolicited injection-site reactions after ProQuad® Dose 1 was determined.

Percentage of Participants Experiencing a Systemic Adverse Event After ProQuad® Dose 1
Up to Day 28 (up to 28 days after ProQuad® Dose 1)

The percentage of participants with systemic adverse events after ProQuad® Dose 1 was determined.

Percentage of Participants With Rectal (or Rectal Equivalent) Temperature ≥ 39.4°C
Up to Day 28 (up to 28 days after ProQuad® Dose 1)

The percentage of participants with a rectal (or rectal equivalent) temperature ≥ 39.4°C after ProQuad® Dose 1 was determined.

Secondary Endpoints

Geometric Mean Titres (GMT) to Measles, Mumps, Rubella, and Varicella After ProQuad® Dose 1
Day 42 (6 weeks after ProQuad® Dose 1)
GMT to Measles, Mumps, Rubella, and Varicella After ProQuad® Dose 2
Day 132 (6 weeks after ProQuad® Dose 2)
Percentage of Participants With Varicella Antibody Titre ≥ 1.25 gpELISA Units/mL
Day 132 (6 weeks after ProQuad® Dose 2)
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Study Design & Arms

AllocationRANDOMIZED
MaskingNONE
ModelPARALLEL
PurposePREVENTION

Treatment Arms

ArmTypeDescription
Arm 1: ProQuad® at 9 and 12 monthsEXPERIMENTALPediatric participants received ProQuad® Dose 1 at 9 months of age and ProQuad® Dose 2 at 12 months of age.
Arm 2: ProQuad® at 11 and 14 monthsEXPERIMENTALPediatric participants received ProQuad® Dose 1 at 11 months of age and ProQuad® Dose 2 at 14 months of age.
Arm 3: ProQuad at 12 and 15 monthsACTIVE_COMPARATORPediatric participants received ProQuad® Dose 1 at 12 months of age and ProQuad® Dose 2 at 15 months of age.

Interventions

NameTypeDescription
ProQuad® manufactured with recombinant Human Albumin (rHA)BIOLOGICALA 2-dose regimen of ProQuad® (0.5 mL per dose) given via subcutaneous injection into the deltoid muscle at a 3-month interval. Each dose contains measles virus Enders' Edmonston strain (live attenuated), mumps virus Jeryl Lynn™ (Level B) strain (live attenuated), rubella virus Wistar RA 27 or 3 strain (live attenuated), and varicella virus Oka or Merck strain (live attenuated).
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Eligibility Criteria

Age Range9 Months to N/A
SexALL
Healthy VolunteersYes

Inclusion Criteria: 1. Healthy subject of either gender of 9 months of age 2. Negative clinical history of measles, mumps, rubella, varicella or zoster 3. Informed consent form signed by both parents or legal representative 4. Parent(s) or legal representative able to attend all the scheduled visit...

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