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PfSPZ Challenge

Phase 1

Healthy | Monoclonal antibody | Other |Merck & Company, Inc.|Last Updated: May 1, 2024

Success Probability

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials1
Total Enrollment39

FDA Designations

No designations recorded

Clinical trial landscape

PfSPZ Challenge · 1 trial · 1 indication

Phase 1 1
NCT04250363Chemoprophylactic Activity of M5717 in PfSPZ Challenge ModelHealthy
COMPLETED39 Analytics
PHASE1COMPLETED
Chemoprophylactic Activity of M5717 in PfSPZ Challenge Model
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Study Endpoints

Primary Endpoints

Early Liver Stage: Number of Participants Over Time With Positive Parasitemia
Early Liver Stage: From Day 1 up to Day 28

Number of participants with positive parasitemia defined as first positive quantitative polymerase chain reaction (qPCR) outcome equal or greater than 100 asexual parasites per milliliter (mL) of blood within 28 days of PfSPZ challenge.

Late Liver Stage: Number of Participants Over Time With Positive Parasitemia
Late Liver Stage: From Day 5 up to Day 32

Number of participants with positive parasitemia defined as first positive quantitative polymerase chain reaction (qPCR) outcome equal or greater than 100 asexual parasites per milliliter (mL) of blood within 28 days of PfSPZ challenge.

Early Liver Stage: Time to First Positive Parasitemia Based on Quantitative Polymerase Chain Reaction (qPCR)
Early Liver Stage: From Day 1 up to Day 28

Time to first positive parasitemia was defined as the time (i.e., number of days) from the PfSPZ DVI (i.e., date of DVI PfSPZ) to the first qPCR outcome greater than or equal to (\>=) 100 asexual parasites per milliliter (mL) of blood.

Late Liver Stage: Time to First Positive Parasitemia Based on Quantitative Polymerase Chain Reaction (qPCR)
Late Liver Stage: From Day 5 up to Day 32

Time to first positive parasitemia was defined as the time (i.e., number of days) from the PfSPZ DVI (i.e., date of DVI PfSPZ) to the first qPCR outcome greater than or equal to (\>=) 100 asexual parasites per milliliter (mL) of blood.

Early Liver Stage: Number of Participants With Documented Blood Stage Parasite Growth
Early Liver Stage: From Day 1 up to Day 28

Documented blood stage parasite growth was defined as an increase of qPCR measured asexual parasites per mL compared to the first parasitemia measurement, within 28 days of PfSPZ DVI.

Late Liver Stage: Number of Participants With Documented Blood Stage Parasite Growth
Late Liver Stage: From Day 5 up to Day 32

Documented blood stage parasite growth was defined as an increase of qPCR measured asexual parasites per mL compared to the first parasitemia measurement, within 28 days of PfSPZ DVI.

Early Liver Stage: Clinical Symptoms of Malaria Assessed Using Malaria Clinical Score
Early Liver Stage: At Day 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 24, 26 and 28

The malaria clinical score consisted of 14 signs/symptoms frequently associated with malaria and graded using a 4-point scale (absent: 0; mild: 1; moderate: 2; severe: 3) and summed to generate a total malaria clinical score (maximum score possible is 42): headache, myalgia (muscle ache), arthralgia (joint ache), fatigue/lethargy, malaise (general discomfort/uneasiness), chills/shivering/rigors, sweating/hot spells, anorexia, nausea, vomiting, abdominal discomfort, fever, tachycardia and hypotension. The minimum score was 0 (no symptoms) and the maximum score was 42 (maximum symptoms). Total scores are reported here.

Late Liver Stage: Clinical Symptoms of Malaria Assessed Using Malaria Clinical Score
Late Liver Stage: At Day 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 26, 28, 30 and 32

The malaria clinical score consisted of 14 signs/symptoms frequently associated with malaria and graded using a 4-point scale (absent: 0; mild: 1; moderate: 2; severe: 3) and summed to generate a total malaria clinical score (maximum score possible is 42): headache, myalgia (muscle ache), arthralgia (joint ache), fatigue/lethargy, malaise (general discomfort/uneasiness), chills/shivering/rigors, sweating/hot spells, anorexia, nausea, vomiting, abdominal discomfort, fever, tachycardia and hypotension. The minimum score was 0 (no symptoms) and the maximum score was 42 (maximum symptoms). Total scores are reported here.

Dose Exposure Response Relationship of M5717 Assessed by Logistic Regression Model
From Day 5 up to Day 32

Exposure efficacy relationship was analyzed using logistic regression model. Different exposure matrices (AUC0-24, AUC0-168, AUC0-inf, C24 and C168) were analyzed using logistic regression model.

Secondary Endpoints

Number of Participants With Treatment-emergent Adverse Events (TEAEs), Serious TEAEs and Treatment-related TEAEs
Early Liver Stage: From Day 1 up to Day 33; Late Liver Stage: From Day 1 up to Day 36
Number of Participants With Treatment Emergent Adverse Events (TEAEs) Based on Severity
Early Liver Stage: From Day 1 up to Day 33; Late Liver Stage: From Day 1 up to Day 36
Number of Participants With Clinically Significant Change From Baseline in Laboratory Values
Early Liver Stage: From Day 1 up to Day 33; Late Liver Stage: From Day 1 up to Day 36
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Study Design & Arms

AllocationRANDOMIZED
MaskingDOUBLE
ModelSEQUENTIAL
PurposePREVENTION

Treatment Arms

ArmTypeDescription
Early liver stage: Pooled PlaceboPLACEBO_COMPARATORParticipants received single dose of placebo matched to M5717 capsule on Day 1 after 2 hours of Plasmodium falciparum Sporozoite (PfSPZ) (3200 sporozoites per injection) intravenous (IV) inoculation administration on Day 1.
Early liver stage: 30 mg M5717EXPERIMENTALParticipants received single dose of M5717 30 mg capsule on Day 1 after 2 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Early liver stage: 60 mg M5717EXPERIMENTALParticipants received single dose of M5717 60 mg capsule on Day 1 after 2 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Early liver stage: 80 mg M5717EXPERIMENTALParticipants received single dose of M5717 80 mg capsule on Day 1 after 2 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Early liver stage: 100 mg M5717EXPERIMENTALParticipants received single dose of M5717 100 mg capsule on Day 1 after 2 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Early liver stage: 200 mg M5717EXPERIMENTALParticipants received single dose of M5717 200 mg capsule on Day 1 after 2 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Late liver stage: Pooled PlaceboEXPERIMENTALParticipants received single dose of placebo matched to M5717 capsule on Day 5 after 96 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Late liver stage: 60 mg M5717EXPERIMENTALParticipants received single dose of M5717 60 mg capsule on Day 5 after 96 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Late liver stage: 100 mg M5717EXPERIMENTALParticipants received single dose of M5717 100 mg capsule on Day 5 after 96 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.
Late liver stage: 200 mg M5717EXPERIMENTALParticipants received single dose of M5717 200 mg capsule on Day 5 after 96 hours of PfSPZ (3200 sporozoites per injection) IV inoculation administration on Day 1.

Interventions

NameTypeDescription
PfSPZ ChallengeBIOLOGICALParticipants received 3200 units of Plasmodium falciparum sporozoites by DVI on Day 1.
PalceboDRUGParticipants received single oral dose of placebo matched to M5717 capsule on Day 1.
M5717 30 mgDRUGParticipants received 30 mg single oral dose of M5717 capsule on Day 1.
M5717 60 mgDRUGParticipants received 60 mg single oral dose of M5717 capsule on Day 1.
M5717 80 mgDRUGParticipants received 80 mg single oral dose of M5717 capsule on Day 1.
M5717 100 mgDRUGParticipants received 100 mg single oral dose of M5717 capsule on Day 1.
M5717 200 mgDRUGParticipants received 200 mg single oral dose of M5717 capsule on Day 1.
PlaceboDRUGParticipants received single oral dose of placebo matched to M5717 capsule on Day 5.
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Eligibility Criteria

Age Range18 Years to 45 Years
SexALL
Healthy VolunteersYes
Study Sites1

Inclusion Criteria: * Participants who are overtly healthy as determined by medical evaluation, including no clinically significant abnormality identified on physical examination or laboratory evaluation and no active clinically significant disorder, condition, infection or disease that would pose ...

Countries:Netherlands
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Frequently asked questions about PfSPZ Challenge

What is PfSPZ Challenge used for?

PfSPZ Challenge is an investigational drug being studied in healthy volunteers. It is being evaluated in a Phase 1 clinical trial for its chemoprophylactic activity, meaning it is tested for its ability to prevent malaria infection. The drug is currently in clinical development and is not approved for any indication.

Who makes PfSPZ Challenge?

PfSPZ Challenge is being developed by Merck & Company, Inc., a pharmaceutical company traded on the New York Stock Exchange under the ticker symbol MRK. The company is conducting clinical research on this investigational drug in healthy volunteers.

What phase is PfSPZ Challenge in?

PfSPZ Challenge is in Phase 1 clinical development. It is an investigational drug, meaning it has not been approved by regulatory authorities. The drug is being studied in a single completed Phase 1 trial that enrolled 39 healthy participants.

What clinical trials is PfSPZ Challenge in?

PfSPZ Challenge has been studied in one clinical trial with the identifier NCT04250363, titled "Chemoprophylactic Activity of M5717 in PfSPZ Challenge Model." This Phase 1 trial was completed and enrolled 39 healthy volunteers in the Netherlands. The trial was randomized, double-blind, and placebo-controlled.

Is PfSPZ Challenge a monoclonal antibody?

PfSPZ Challenge is classified as a monoclonal antibody modality. It is being investigated in a clinical trial that is randomized, double-blind, and placebo-controlled, with healthy volunteers as participants. The drug is in Phase 1 development for prophylactic use.