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Monovalent MRKAd5 HIV-1 gag vaccine

Phase 1

HIV-1 | Monoclonal antibody | Infectious Disease |Merck & Company, Inc.|Last Updated: Feb 2, 2015

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials1
Total Enrollment317

FDA Designations

No designations recorded

Clinical trial landscape

Monovalent MRKAd5 HIV-1 gag vaccine · 1 trial · 2 indications

Phase 1 1
NCT00849680A Study of Safety, Tolerability, and Immunogenicity of the MRKAd5 Gag/Pol/Nef Vaccine in Healthy Adults (V520-016)HIV-1
COMPLETED317 Analytics
PHASE1COMPLETED
A Study of Safety, Tolerability, and Immunogenicity of the MRKAd5 Gag/Pol/Nef Vaccine in Healthy Adults (V520-016)
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Study Endpoints

Primary Endpoints

Number of Participants With Adverse Experiences
up to 260 weeks after first vaccination

Adverse experiences (AE) collected include serious and non serious systemic AEs, and injection-site AEs. Systemic and Laboratory AEs include any unfavorable \& unintended change in the structure, function, or chemistry of the body. Injection-site AEs include any swelling, redness, pain or tenderness at the injection site. All injection site AEs were collected up to 5 days after any vaccine dose.

Number of Participants With Laboratory Adverse Experiences
up to 260 weeks after first vaccination

Laboratory AEs were based on a grading system considering the severity of abnormal laboratory values in participants and reflect any unfavorable and unintentional change in function, or chemistry of the body. All laboratory AEs were collected up to 29 days after any vaccine dose.

Immune Response by Levels of Unfractionated Gag, Pol, and Nef-specific IFN-gamma Following a 3-dose Vaccine Regimen
4 weeks after booster injection

Participants expressing HIV antigens (gag, pol and nef) secrete antigen specific interferon-gamma (IFN-gamma). Levels of unfractionated gag, pol, and nef-specific IFN-gamma were to be measured using an Enzyme Linked Immunospot Assay (ELISPOT), which measures spot forming cells per 10\^6 peripheral blood mononuclear cells (SFC per million PBMCs).

Immune Response by Levels of Unfractionated Gag, Pol, and Nef-specific IFN-gamma Following a 2-dose Vaccine Regimen
4 weeks after booster injection

Participants expressing HIV antigens (gag, pol and nef) secrete antigen specific interferon-gamma (IFN-gamma). Levels of unfractionated gag, pol, and nef-specific IFN-gamma were to be measured using an Enzyme Linked Immunospot Assay (ELISPOT), which measures spot forming cells per 10\^6 peripheral blood mononuclear cells (SFC per million PBMCs). No immunogenicity analyses were performed because the results from a previous study, V520-023 (NCT00095576), which used the same vaccine as the one used in this study (NCT00849680) proved it was not efficacious.

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Study Design & Arms

AllocationRANDOMIZED
MaskingDOUBLE
ModelPARALLEL
PurposePREVENTION

Treatment Arms

ArmTypeDescription
PlaceboPLACEBO_COMPARATORParticipants receiving 1.0 ml of placebo to the MRKAd5 HIV-1 gag/pol/nef vaccine or the placebo to the MRKAd5 HIV-1 gag vaccine injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26 or a 2-dose regimen at Day 1 and Week 26 or Day 1 and Week 4.
Monovalent MRKAd5 HIV-1 gag vaccine (1x10^9 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of the Monovalent MRKAd5 HIV-1 gag vaccine (1x10\^9 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^6 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^6 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^7 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^7 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^8 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^8 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^9 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^9 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^10 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^10 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26, or in a 2-dose regimen at Day 1 and Week 4 (with no vaccine administered at Week 26) or Day 1 and Week 26 (with placebo to the MRKAd5 HIV-1 gag/pol/nef vaccine administered at Week 4)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (1x10^11 vp/dose)EXPERIMENTALParticipants receiving 1.0 ml of Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (1x10\^11 vp/dose) injected intramuscularly in a 3-dose regimen at Day 1, Week 4 and Week 26.

Interventions

NameTypeDescription
Comparator: Placebo to the MRKAd5 HIV-1 gag/pol/nef vaccineOTHERPlacebo to the MRKAd5 HIV-1 gag/pol/nef vaccine.
Monovalent MRKAd5 HIV-1 gag vaccine (1x10^9 vp/dose)BIOLOGICALMonovalent MRKAd5 HIV-1 gag vaccine (1x10\^9 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^6 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^6 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^7 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^7 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^8 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^8 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^9 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^9 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10^10 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (3x10\^10 vp/dose)
Trivalent MRKAd5 HIV-1 gag/pol/nef vaccine (1x10^11 vp/dose)BIOLOGICALTrivalent MRKAd5 HIV-1 gag/pol/nef vaccine (1x10\^11 vp/dose)
Comparator: Placebo to MRKAd5 HIV-1 gag vaccineBIOLOGICALPlacebo to the MRKAd5 HIV-1 gag vaccine.
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Eligibility Criteria

Age Range18 Years to 45 Years
SexALL
Healthy VolunteersYes

Inclusion Criteria: * Subjects 18 Years to 45 Years in Stages I-III and 18 Years to 50 Years in Stage IV. * Subject is in good general health * Subjects of reproductive potential agree to use acceptable method of birth control through study * Subject tests negative for Hepatitis B, Hepatitis C, and...

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