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VGX-3100

Phase 3

Cervical Dysplasia | Monoclonal antibody | Oncology |Inovio Pharmaceuticals, Inc.|Last Updated: Oct 17, 2024

Target and mechanism

ModalityMonoclonal antibody

Also known as VGX 3100

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLEDDMC
Total Trials2
Total Enrollment404

FDA Designations

No designations recorded

Clinical trial landscape

VGX-3100 · 7 trials · 13 indications

Phase 3 2Phase 2 3Phase 1 2
NCT03721978REVEAL 2 Trial (Evaluation of VGX-3100 and Electroporation for the Treatment of Cervical HSIL)Cervical Dysplasia
COMPLETED203 Analytics
NCT03185013REVEAL 1 (Evaluation of VGX-3100 and Electroporation for the Treatment of Cervical HSIL)Cervical Dysplasia
COMPLETED201 Analytics
PHASE3COMPLETED
REVEAL 2 Trial (Evaluation of VGX-3100 and Electroporation for the Treatment of Cervical HSIL)
Cervical DysplasiaUnlock trial analytics
PHASE3COMPLETED
REVEAL 1 (Evaluation of VGX-3100 and Electroporation for the Treatment of Cervical HSIL)
Cervical DysplasiaUnlock trial analytics

Study Endpoints

Primary Endpoints

Baseline Biomarker-positive Participants for ITT Population: Percentage of Participants With No Histologic Evidence of Cervical HSIL on Histology Sample and No Evidence of HPV-16 and/or HPV-18 in Cervical Samples
At Week 36

Baseline biomarker-positive participants with no histologic (i.e., biopsies or excisional treatment) evidence of cervical HSIL, no evidence of HPV-16 and/or HPV-18 at Week 36, and participants who did not have unscheduled excision or biopsy sample obtained between the initial dose up to Week 36 were considered to be responders. No evidence of HSIL was defined by histology as negative, squamous atypia, or low-grade intraepithelial lesion (LSIL). Cervical samples for HPV-16 and/or HPV-18 were collected using the ThinPrep™. The efficacy time frame is defined by a biopsy or surgical excision at any time starting from 14 days prior to the protocol-specified target date of Week 36. The first tissue removal sample within the time frame determines the histology endpoint.

Percentage of Participants With No Evidence of Cervical HSIL on Histology and No Evidence of HPV-16 and/or HPV-18 in Cervical Samples
Week 36

Participants with no histologic (i.e., biopsies or excisional treatment) evidence of cervical HSIL, no evidence of HPV-16 and/or HPV-18 at the Week 36 time frame, and participants in which excision or biopsy sample was not obtained between the initial dose up to Week 36 were considered to be responders. No evidence of HSIL was defined by histology as negative, squamous atypia, or low-grade intraepithelial lesion (LSIL). Cervical samples for HPV-16 and/or HPV-18 were collected using the ThinPrep®.

Percentage of Participants With no Histologic Evidence of Anal or Anal/Peri-Anal HSIL and no Evidence of HPV-16/18 at Week 36
Week 36
Percentage of Participants With No Histologic Evidence of Vulvar HSIL and No Evidence of HPV-16 and/or HPV-18 in Vulvar Tissue Samples
Week 48

A treatment responder for the primary endpoint was defined as a participant with no histologic evidence of vulvar HSIL (normal tissue or vulvar low grade squamous intraepithelial lesions (LSIL) \[vulval intraepithelial neoplasia 1 (VIN1)\] or condyloma) and no evidence of HPV-16 or HPV-18 (i.e., elimination of the specific genotypes \[16, 18, or both\]) in vulvar tissue at evaluation and who did not receive any non-study related treatment for vulvar HSIL. All lesions were evaluated for histologic evidence of vulvar HSIL or evidence of HPV-16/18 in vulvar tissue.

Number of Participants with Histopathological Regression of Cervical Lesions to CIN 1 or Less as a Measure of Efficacy.
36 weeks

The number of participants with histopathologically confirmed CIN2/3 or CIN 3 associated with HPV16 or HPV18 whose cervical lesions regress to CIN 1 or less at the 36 week visit.

Safety and tolerability of a fourth dose of VGX-3100
through Month 6 (end of study)

Safety and tolerability of a fourth dose of VGX-3100, administered by IM injection with EP to adult females who have been previously immunized with three doses of VGX-3100

Safety and tolerability of escalating doses of VGX-3100, administered by IM injection with EP to adult female subjects post surgical or ablative treatment of grade 2 or 3 CIN.
Through Month 4

Secondary Endpoints

Baseline Biomarker-positive Participants for Safety Population: Number of Participants With Any Local and Systemic Adverse Events (AEs)
From Baseline to Week 40
Safety Population: Number of Participants With Local and Systemic AEs
From Baseline to Week 40
Baseline Biomarker-positive Participants for Safety Population: Number of Participants With Any Treatment Emergent AEs (TEAEs) and Serious TEAEs (Including Suspected Unexpected Serious Adverse Reaction [SUSAR] and Unexpected Adverse Device Effect [UADE])
From Baseline to Week 40
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Study Design & Arms

AllocationRANDOMIZED
MaskingQUADRUPLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
VGX-3100 + EPEXPERIMENTALParticipants received 3 IM injections of 6 mg (in 1 mL) VGX-3100 followed by EP using the CELLECTRA™-5PSP device on Day 0, Week 4, and Week 12.
Matched Placebo + EPPLACEBO_COMPARATORParticipants received 3 IM injections of 1 mL VGX-3100 matching placebo followed by EP using the CELLECTRA™-5PSP device on Day 0, Week 4, and Week 12.
Placebo + EPPLACEBO_COMPARATORParticipants received three IM injections of 1 mL VGX-3100 matching placebo followed by EP using the CELLECTRA™-5PSP device on Day 0, Week 4, and Week 12.
VGX-3100EXPERIMENTALAdult participants who were HIV negative with histologically confirmed anal or anal/peri-anal HSIL associated with HPV-16 and/or 18, received four 6 mg doses of VGX-3100 as an IM injection on Day 0, Week 4, Week 12, and Week 40 followed immediately by EP using the CELLECTRA™ 5PSP device.
VGX-3100 + EP + ImiquimodEXPERIMENTALParticipants with histologically confirmed vulvar HSIL associated with HPV-16 and/or 18, received four doses of 6 mg VGX-3100 as an IM injection on Day 0, Week 4, Week 12, and Week 24 followed by EP using the CELLECTRA™ 2000 device. Participants with vulvar HSIL who had a reduction in lesion size or no increase in lesion size at Week 48, received a fifth dose of VGX-3100 at Week 52. In addition, participants applied imiquimod 5% cream to the vulvar lesion three times per week for 20 weeks.
VGX 3100EXPERIMENTAL -
PlaceboPLACEBO_COMPARATOR -
6mg of DNA/doseEXPERIMENTALSubjects who have previously received a 3 dose series of VGX-3100 containing either 0.6, 2 or 6mg DNA/dose will receive a fourth dose of VGX-3100 containing 6mg of DNA/dose administered via IM injection + electroporation at Day 0
0.6mg of DNA/doseEXPERIMENTALSubjects will receive a 3 dose series of VGX-3100 containing 0.6mg DNA/dose administered via IM injection + electroporation at Day 0, Month 1 and Month 3
2mg of DNA/doseEXPERIMENTALSubjects will receive a 3 dose series of VGX-3100 containing 2mg of DNA/dose administered via IM injection + electroporation at Day 0, Month 1 and Month 3

Interventions

NameTypeDescription
VGX-3100BIOLOGICAL1 milliliter (mL) VGX-3100 injected IM.
Matched PlaceboBIOLOGICAL1 mL of matched Placebo injected IM.
CELLECTRA™-5PSPDEVICECELLECTRA™-5PSP used for EP following IM injection of VGX 3100.
PlaceboBIOLOGICAL1 mL of Placebo will be injected IM and delivered by EP using CELLECTRA™-5PSP on Day 0, Week 4 and Week 12.
Electroporation (EP)DEVICEIntramuscular injection followed by EP with the CELLECTRA™ 5PSP device.
CELLECTRA™ 5PSPDEVICEIM injection of VGX-3100 is followed by EP with the CELLECTRA™ 5PSP device.
Imiquimod 5% CreamDRUGParticipants applied imiquimod 5% cream to the vulvar lesion three times per week for 20 weeks.
CELLECTRA™ 2000DEVICEIM injection of VGX-3100 was followed by EP with the CELLECTRA™ 2000 device.
VGX 3100BIOLOGICAL1ml of VGX-3100 delivered IM followed by electroporation at Day 0, Week 4 and Week 12.
CELLECTRA™-5PDEVICECELLECTRA™-5P is used for electroporation following IM delivery of VGX 3100 or placebo on Day 0, Week 4 and Week 12.
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Eligibility Criteria

Age Range18 Years to N/A
SexFEMALE
Healthy VolunteersNo
Study Sites53

Inclusion Criteria: * Women aged 18 years and above * Confirmed cervical infection with HPV types 16 and/or 18 at screening * Cervical tissue specimen/slides provided to Study Pathology Adjudication Committee for diagnosis scheduled to be collected within 10 weeks prior to anticipated date of first...

Countries:United StatesArgentinaBrazilEstoniaFinlandLithuaniaPolandPuerto RicoSouth AfricaSpainBelgiumGermanyItalyMexicoPeruPhilippinesPortugalSlovakiaThailandUnited KingdomCanadaAustraliaGeorgiaIndiaSouth Korea
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Frequently asked questions about VGX-3100

What is VGX 3100 used for?

VGX 3100 is an investigational immunotherapy being developed for the treatment of high-grade squamous intraepithelial lesions (HSIL) caused by human papillomavirus (HPV), including cervical intraepithelial neoplasia (CIN) and anal HSIL. It is being studied in patients with HPV-16 and/or HPV-18 related cervical and anal precancerous lesions.

Who makes VGX 3100?

VGX 3100 is being developed by Inovio Pharmaceuticals, Inc., a biopharmaceutical company traded on the NASDAQ under the ticker symbol INO. The company is conducting clinical trials to evaluate the safety and efficacy of VGX 3100 for the treatment of HPV-related precancerous conditions.

What phase is VGX 3100 in?

VGX 3100 is currently in Phase 3 clinical development. It has completed Phase 2 and Phase 3 trials, including the REVEAL 1 and REVEAL 2 studies, which evaluated the treatment of cervical HSIL. The drug is investigational and has not been approved by regulatory authorities.

What clinical trials is VGX 3100 in?

VGX 3100 has been studied in several clinical trials, including NCT01304524 (Phase 2, cervical intraepithelial neoplasia), NCT03185013 (REVEAL 1, Phase 3, cervical HSIL), NCT03499795 (Phase 2, anal HSIL), and NCT03721978 (REVEAL 2, Phase 3, cervical HSIL). All trials have been completed.

How does VGX 3100 work?

VGX 3100 is a DNA vaccine that targets HPV-16 and HPV-18 antigens. It is designed to generate an immune response against these viral proteins, which are responsible for the development of precancerous lesions. The vaccine is delivered intramuscularly followed by electroporation to enhance cellular uptake and immune activation.

Is VGX 3100 the same as VGX-3100?

Yes, VGX 3100 and VGX-3100 refer to the same investigational drug. The name is used interchangeably in clinical trial registrations and scientific literature. Both names denote the DNA vaccine being developed by Inovio Pharmaceuticals for the treatment of HPV-related high-grade squamous intraepithelial lesions.