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PrabotulinumtoxinA-Xvfs

Phase 2

Glabellar Frown Lines | Small molecule | Dermatology |Evolus, Inc. Common Stock|Last Updated: Oct 21, 2025

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Trial Design

RandomizedDouble-BlindACTIVE_CONTROLLEDBiomarker
Total Trials1
Total Enrollment154

FDA Designations

No designations recorded

Clinical trial landscape

PrabotulinumtoxinA-Xvfs · 3 trials · 4 indications

Phase 2 1Phase 1 1Early Phase 1 1
NCT05320393Study to Demonstrate Safety and Duration of Effect of 40 Units of PrabotulinumtoxinA-xvfs to Treat Glabellar LinesGlabellar Frown Lines
COMPLETED154 Analytics
PHASE2COMPLETED
Study to Demonstrate Safety and Duration of Effect of 40 Units of PrabotulinumtoxinA-xvfs to Treat Glabellar Lines
Glabellar Frown LinesUnlock trial analytics

Study Endpoints

Primary Endpoints

Duration of Effect Described by Kaplan-Meier Analysis
Duration of effect for each subject will be based on number of days from baseline to the day that Glabellar Line Scale severity (Investigator Assessment) returns to baseline value, assessed every 30 days up to 365 days.

Effectiveness of prabotulinumtoxinA 40 Units compared to prabotulinumtoxinA 20 Units will be assessed for each subject based on the number of days from baseline to the day that the Glabellar Line Scale (GLS) severity, by Investigator Assessment, returns to the baseline value. The treatment will be considered to remain effective for the amount of time during which the GLS severity at maximum frown has not returned to baseline severity.

Scientific Assessment Scale of Skin Quality (SASSQ) Rating
Up to 12 weeks

The treating physician and the blinded, secondary evaluator will rate the subject after the final treatment. The scale identifies 6 parameters of skin quality: (1) loss of elasticity, (2) wrinkles, (3) roughness, (4) pigmentation/lentigines, (5) erythema, and (6) pore size. The scale has 5 points to describe the intensity of parameters 1-5: 0 implies none, 1 implies mild, 2 implies moderate, 3 implies severe, and 4 implies very severe. For parameter 6, 0 implies fine, 1 implies small, 2 implies moderate, 3 implies large, and 4 implies very large.

Global Aesthetic Improvement Scale (GAIS) Rating
Up to 12 weeks

The treating physician and the blinded, secondary evaluator will rate the subject after the final treatment. Based on aesthetic appearance, qualitative success of treatment will be evaluated using the GAIS. Aesthetic appearance will be rated one of the five following options: - Worse: The appearance is worse than the original condition - No Change: The appearance is essentially the same as the original condition - Improved: Obvious improvement in appearance from the initial condition, but a touchup or re-treatment is indicated - Much Improved: Marked improvement in appearance but not completely optimal for this patient. A touchup would slightly improve the result - Very Much Improved: Optimal cosmetic result for the treatment in this patient.

Surface area of rhytid diminution
14 days post-treatment

Primary endpoint is surface area of rhytid diminution. Surface area of rhytid reduction will be measured in centimeters squared (incorporating length and width measurements) two-weeks post-injection to assess differences, if any, in rhytid reduction on each side of the forehead by the blinded investigator.

Secondary Endpoints

Scientific Assessment Scale of Skin Quality (SASSQ) Rating
Up to 2 weeks
Global Aesthetic Improvement Scale (GAIS) Rating
Up to 2 weeks
Patient Satisfaction with Treatment and Aesthetic Appearance
Up to 12 weeks
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Study Design & Arms

AllocationRANDOMIZED
MaskingDOUBLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
Study DrugEXPERIMENTAL40U of PrabotulinumtoxinA-xvfs
OnabotulinumtoxinAACTIVE_COMPARATOR20U of OnabotulinumtoxinA
PrabotulinumtoxinA-xvfsACTIVE_COMPARATOR20U of PrabotulinumtoxinA-xvfs
Treated with prabotulinumtoxinA-xvfs and placeboPLACEBO_COMPARATORSubjects will have one treatment session at week 0 and two live assessment visits at weeks 2 and 12. Approximately 20 units of prabotulinumtoxinA-xvfs will be injected into the left side of the subject's glabellar lines and forehead region, and a placebo saline treatment will be injected into the right side of the subject's glabellar lines and forehead region.
Treated with prabotulinumtoxinA-xvfs and incobotulinumtoxinAACTIVE_COMPARATORSubjects will have one treatment session at week 0 and two live assessment visits at weeks 2 and 12. Approximately 20 units of prabotulinumtoxinA-xvfs will be injected into the left side of the subject's glabellar lines and forehead region, and approximately 20 units of incobotulinumtoxinA will be injected into the right side of the subject's glabellar lines and forehead region.
Experimental halfEXPERIMENTALEach subject will be randomized to have either their right forehead or left forehead treated with 4 units or 0.1 cc of prabotulinumtoxinA 2.5 cm above the orbital rim.
Comparator halfACTIVE_COMPARATOREach subject will be randomized to have either their right forehead or left forehead treated with 4 units or 0.1 cc of onabotulinumtoxinA 2.5 cm above the orbital rim.

Interventions

NameTypeDescription
PrabotulinumtoxinA-XvfsDRUGOne treatment of 40 units
OnabotulinumtoxinADRUGOne treatment of 20 units
IncobotulinumtoxinADRUGEach vial of incobotulinumtoxinA contains 100 units of product, and it will be reconstituted with 5 mL of bacteriostatic saline solution. 20 units of this intervention will only be injected to the right side of subject faces - intramuscularly to the corrugator supercilii and intradermally to the forehead region.
saline solution (placebo)DRUGAs a placebo treatment, saline solution will be injected on the right side of the glabellar lines and forehead region instead of an active neuromodulator. The saline solution has no therapeutic effect and is used as a control to compare the results against the active treatment.
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Eligibility Criteria

Age Range18 Years to N/A
SexALL
Healthy VolunteersYes
Study Sites1

Inclusion Criteria: * Outpatient, male or female of any race, 18 years of age or older. * Female subjects of childbearing potential must have a negative urine pregnancy test during Visits 1 (Screening) and Visit 2 (if applicable) and practice a reliable method of contraception for the duration of t...

Countries:United States
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Frequently asked questions about PrabotulinumtoxinA-Xvfs

What is PrabotulinumtoxinA-Xvfs used for?

PrabotulinumtoxinA-Xvfs is an investigational botulinum toxin type A product being studied for cosmetic dermatology uses, including glabellar frown lines, forehead rhytides, and skin aging. It is being developed by Evolus, Inc. (EOLS) and is currently in Phase 2 clinical development for these indications.

What does PrabotulinumtoxinA-Xvfs target?

PrabotulinumtoxinA-Xvfs is a botulinum toxin type A product that works by temporarily relaxing facial muscles that cause wrinkles. It is being studied for its effects on glabellar frown lines, forehead rhytides, and overall skin quality, including fine wrinkles and texture.

Who makes PrabotulinumtoxinA-Xvfs?

PrabotulinumtoxinA-Xvfs is being developed by Evolus, Inc., a company traded on the stock exchange under the ticker symbol EOLS. Evolus is focused on aesthetic medicine and is conducting clinical trials to evaluate the safety and efficacy of this product for cosmetic indications.

What phase is PrabotulinumtoxinA-Xvfs in?

PrabotulinumtoxinA-Xvfs is in Phase 2 clinical development. The most advanced completed trial, NCT05320393, was a Phase 2 study evaluating 40 units of the product for treating glabellar lines. The product is investigational and has not been approved by regulatory authorities.

What clinical trials is PrabotulinumtoxinA-Xvfs in?

PrabotulinumtoxinA-Xvfs has been studied in three completed clinical trials. NCT05320393 was a Phase 2 study in 154 participants with glabellar frown lines. NCT05807412 was an early Phase 1 study in 10 participants with forehead rhytides. NCT06604832 was a Phase 1 study in 20 female participants with skin aging and wrinkling.

Is PrabotulinumtoxinA-Xvfs the same as Botox?

PrabotulinumtoxinA-Xvfs is not the same as Botox, but it is a similar botulinum toxin type A product. In clinical trials, it has been compared directly with onabotulinumtoxinA, which is the active ingredient in Botox, to assess differences in spread and duration of effect for treating forehead rhytides.