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PrabotulinumtoxinA-Xvfs

Phase 2

Glabellar Frown Lines | Small molecule | Dermatology |Evolus, Inc. Common Stock|Last Updated: Oct 21, 2025

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Trial Design

RandomizedDouble-BlindACTIVE_CONTROLLEDBiomarker
Total Trials1
Total Enrollment154

FDA Designations

No designations recorded

Clinical trial landscape

PrabotulinumtoxinA-Xvfs · 3 trials · 4 indications

Phase 2 1Phase 1 1Early Phase 1 1
NCT05320393Study to Demonstrate Safety and Duration of Effect of 40 Units of PrabotulinumtoxinA-xvfs to Treat Glabellar LinesGlabellar Frown Lines
COMPLETED154 Analytics
PHASE2COMPLETED
Study to Demonstrate Safety and Duration of Effect of 40 Units of PrabotulinumtoxinA-xvfs to Treat Glabellar Lines
Glabellar Frown LinesUnlock trial analytics

Study Endpoints

Primary Endpoints

Duration of Effect Described by Kaplan-Meier Analysis
Duration of effect for each subject will be based on number of days from baseline to the day that Glabellar Line Scale severity (Investigator Assessment) returns to baseline value, assessed every 30 days up to 365 days.

Effectiveness of prabotulinumtoxinA 40 Units compared to prabotulinumtoxinA 20 Units will be assessed for each subject based on the number of days from baseline to the day that the Glabellar Line Scale (GLS) severity, by Investigator Assessment, returns to the baseline value. The treatment will be considered to remain effective for the amount of time during which the GLS severity at maximum frown has not returned to baseline severity.

Scientific Assessment Scale of Skin Quality (SASSQ) Rating
Up to 12 weeks

The treating physician and the blinded, secondary evaluator will rate the subject after the final treatment. The scale identifies 6 parameters of skin quality: (1) loss of elasticity, (2) wrinkles, (3) roughness, (4) pigmentation/lentigines, (5) erythema, and (6) pore size. The scale has 5 points to describe the intensity of parameters 1-5: 0 implies none, 1 implies mild, 2 implies moderate, 3 implies severe, and 4 implies very severe. For parameter 6, 0 implies fine, 1 implies small, 2 implies moderate, 3 implies large, and 4 implies very large.

Global Aesthetic Improvement Scale (GAIS) Rating
Up to 12 weeks

The treating physician and the blinded, secondary evaluator will rate the subject after the final treatment. Based on aesthetic appearance, qualitative success of treatment will be evaluated using the GAIS. Aesthetic appearance will be rated one of the five following options: - Worse: The appearance is worse than the original condition - No Change: The appearance is essentially the same as the original condition - Improved: Obvious improvement in appearance from the initial condition, but a touchup or re-treatment is indicated - Much Improved: Marked improvement in appearance but not completely optimal for this patient. A touchup would slightly improve the result - Very Much Improved: Optimal cosmetic result for the treatment in this patient.

Surface area of rhytid diminution
14 days post-treatment

Primary endpoint is surface area of rhytid diminution. Surface area of rhytid reduction will be measured in centimeters squared (incorporating length and width measurements) two-weeks post-injection to assess differences, if any, in rhytid reduction on each side of the forehead by the blinded investigator.

Secondary Endpoints

Scientific Assessment Scale of Skin Quality (SASSQ) Rating
Up to 2 weeks
Global Aesthetic Improvement Scale (GAIS) Rating
Up to 2 weeks
Patient Satisfaction with Treatment and Aesthetic Appearance
Up to 12 weeks
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Study Design & Arms

AllocationRANDOMIZED
MaskingDOUBLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
Study DrugEXPERIMENTAL40U of PrabotulinumtoxinA-xvfs
OnabotulinumtoxinAACTIVE_COMPARATOR20U of OnabotulinumtoxinA
PrabotulinumtoxinA-xvfsACTIVE_COMPARATOR20U of PrabotulinumtoxinA-xvfs
Treated with prabotulinumtoxinA-xvfs and placeboPLACEBO_COMPARATORSubjects will have one treatment session at week 0 and two live assessment visits at weeks 2 and 12. Approximately 20 units of prabotulinumtoxinA-xvfs will be injected into the left side of the subject's glabellar lines and forehead region, and a placebo saline treatment will be injected into the right side of the subject's glabellar lines and forehead region.
Treated with prabotulinumtoxinA-xvfs and incobotulinumtoxinAACTIVE_COMPARATORSubjects will have one treatment session at week 0 and two live assessment visits at weeks 2 and 12. Approximately 20 units of prabotulinumtoxinA-xvfs will be injected into the left side of the subject's glabellar lines and forehead region, and approximately 20 units of incobotulinumtoxinA will be injected into the right side of the subject's glabellar lines and forehead region.
Experimental halfEXPERIMENTALEach subject will be randomized to have either their right forehead or left forehead treated with 4 units or 0.1 cc of prabotulinumtoxinA 2.5 cm above the orbital rim.
Comparator halfACTIVE_COMPARATOREach subject will be randomized to have either their right forehead or left forehead treated with 4 units or 0.1 cc of onabotulinumtoxinA 2.5 cm above the orbital rim.

Interventions

NameTypeDescription
PrabotulinumtoxinA-XvfsDRUGOne treatment of 40 units
OnabotulinumtoxinADRUGOne treatment of 20 units
IncobotulinumtoxinADRUGEach vial of incobotulinumtoxinA contains 100 units of product, and it will be reconstituted with 5 mL of bacteriostatic saline solution. 20 units of this intervention will only be injected to the right side of subject faces - intramuscularly to the corrugator supercilii and intradermally to the forehead region.
saline solution (placebo)DRUGAs a placebo treatment, saline solution will be injected on the right side of the glabellar lines and forehead region instead of an active neuromodulator. The saline solution has no therapeutic effect and is used as a control to compare the results against the active treatment.
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Eligibility Criteria

Age Range18 Years to N/A
SexALL
Healthy VolunteersYes
Study Sites1

Inclusion Criteria: * Outpatient, male or female of any race, 18 years of age or older. * Female subjects of childbearing potential must have a negative urine pregnancy test during Visits 1 (Screening) and Visit 2 (if applicable) and practice a reliable method of contraception for the duration of t...

Countries:United States
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Frequently asked questions about PrabotulinumtoxinA-Xvfs

What is PrabotulinumtoxinA-Xvfs used for?

PrabotulinumtoxinA-Xvfs is an investigational botulinum toxin type A product being studied for dermatologic uses, including glabellar frown lines, forehead rhytides, and skin aging with fine wrinkles. It is in clinical development by Evolus, Inc. (EOLS) and is not yet approved for these indications.

How does PrabotulinumtoxinA-Xvfs work?

PrabotulinumtoxinA-Xvfs is a botulinum toxin type A that works by blocking nerve signals to muscles, temporarily reducing muscle activity that causes wrinkles. This mechanism underlies its use in treating glabellar lines and forehead rhytides by relaxing the muscles responsible for these expression lines.

Who makes PrabotulinumtoxinA-Xvfs?

PrabotulinumtoxinA-Xvfs is being developed by Evolus, Inc., a company traded on the stock market under the ticker EOLS. Evolus is focused on aesthetic medicine and is conducting clinical trials to evaluate this product for facial wrinkle treatments.

What phase is PrabotulinumtoxinA-Xvfs in?

PrabotulinumtoxinA-Xvfs is in Phase 2 clinical development for glabellar frown lines, based on a completed Phase 2 trial. It has also been studied in earlier-phase trials for forehead rhytides and skin aging. It remains investigational and has not received FDA approval.

What clinical trials is PrabotulinumtoxinA-Xvfs in?

PrabotulinumtoxinA-Xvfs has been studied in three completed trials: NCT05320393, a Phase 2 study of 40 units for glabellar lines with 154 participants; NCT05807412, an early Phase 1 study comparing spread versus onabotulinumtoxinA for forehead rhytides with 10 participants; and NCT06604832, a Phase 1 study of microtox for facial skin quality with 20 female participants.

Is PrabotulinumtoxinA-Xvfs the same as Botox?

PrabotulinumtoxinA-Xvfs is not the same as Botox, though both are botulinum toxin type A products. In a clinical trial, PrabotulinumtoxinA-Xvfs was compared directly with onabotulinumtoxinA, the active ingredient in Botox, to assess differences in spread when treating forehead rhytides. They are distinct products.