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EDIT-301

Phase 1

Sickle Cell Disease | Gene therapy | Hematology |Editas Medicine, Inc.|Last Updated: Apr 2, 2025

Target and mechanism

Molecular targetAsCas12a
Target classGene-editing Enzyme
ModalityGene therapy

Success Probability

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Market & Valuation

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Trial Design

UNCONTROLLEDDMC
Total Trials1
Total Enrollment45

FDA Designations

ORPHAN_DRUGRARE_PEDIATRIC_DISEASERMAT

Clinical trial landscape

EDIT-301 · 2 trials · 5 indications

Phase 1 2
NCT05444894EDIT-301 for Autologous Hematopoietic Stem Cell Transplant (HSCT) in Participants With Transfusion-Dependent Beta Thalassemia (TDT)Transfusion Dependent Beta Thalassemia
ACTIVE NOT_RECRUITING9 Analytics
NCT04853576A Study Evaluating the Safety and Efficacy of EDIT-301 in Participants With Severe Sickle Cell Disease (RUBY)Sickle Cell Disease
ACTIVE NOT_RECRUITING45 Analytics
PHASE1ACTIVE NOT_RECRUITING
EDIT-301 for Autologous Hematopoietic Stem Cell Transplant (HSCT) in Participants With Transfusion-Dependent Beta Thalassemia (TDT)
Transfusion Dependent Beta ThalassemiaUnlock trial analytics
PHASE1ACTIVE NOT_RECRUITING
A Study Evaluating the Safety and Efficacy of EDIT-301 in Participants With Severe Sickle Cell Disease (RUBY)
Sickle Cell DiseaseUnlock trial analytics

Study Endpoints

Primary Endpoints

Proportion of participants achieving engraftment defined as neutrophil engraftment (defined as demonstrating absolute neutrophil count (ANC) ≥ 0.5 x 10^9/L post EDIT-301 infusion for 3 consecutive measurements obtained on different days)
EDIT-301 infusion (Day 0) to 42 days post EDIT-301 infusion
Frequency and severity of adverse events (AEs) (incidence of AEs and Grade 3 or higher serious adverse events, using National Cancer Institute Common Terminology Criteria for Adverse Events [NCI CTCAE] v.5.0)
Screening through up to 24 months post EDIT-301 infusion
Proportion of subjects achieving complete resolution of severe vaso-occlusive events (VOEs)
from Month 6 through Month 18 post EDIT-301 infusion

Secondary Endpoints

Kinetics of HSPC engraftment
EDIT-301 infusion (Day 0) to first day in which 3 consecutive measurements obtained on different days demonstrate ANC ≥ 0.5 x 10^9/L up to 24 months post EDIT-301 infusion
Incidence of transplant related mortality
EDIT-301 infusion (Day 0) through Day 100 post EDIT-301 infusion and from EDIT-301 infusion (Day 0) through 12 months post EDIT-301 infusion
Incidence of all-cause mortality
Screening through up to 24 months post EDIT-301 infusion
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Study Design & Arms

AllocationNA
MaskingNONE
ModelSINGLE_GROUP
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
EDIT-301EXPERIMENTALEDIT-301 (autologous gene edited (CD)34+ hematopoietic stem cells) will be administered as a one-time intravenous infusion.

Interventions

NameTypeDescription
EDIT-301GENETICAdministered by intravenous infusion after myeloablative conditioning with busulfan.
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Eligibility Criteria

Age Range18 Years to 35 Years
SexALL
Healthy VolunteersNo
Study Sites8

Key Inclusion Criteria: Diagnosis of Transfusion Dependent B-Thalassemia as defined by: * Documented homozygous β-thalassemia or compound heterozygous β-thalassemia including β-thalassemia/hemoglobin E (HbE) based on historical data in medical records, and * History of at least 100 mL/kg/year or 1...

Countries:United StatesCanada
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Frequently asked questions about EDIT-301

What is EDIT-301 used for?

EDIT-301 is an investigational gene therapy being developed for transfusion dependent beta thalassemia and sickle cell disease. It is designed to modify a patient's own hematopoietic stem cells to address the underlying genetic cause of these hemoglobin disorders. The therapy is currently in Phase 1 clinical development.

What does EDIT-301 target?

EDIT-301 uses the gene-editing enzyme AsCas12a to make a specific edit in a patient's hematopoietic stem cells. By targeting this enzyme to a particular genetic region, the therapy aims to increase fetal hemoglobin production, which can compensate for defective adult hemoglobin in sickle cell disease and beta thalassemia.

Who is developing EDIT-301?

EDIT-301 is being developed by Editas Medicine, Inc., a biotechnology company traded on the NASDAQ under the ticker symbol EDIT. The company is conducting clinical trials to evaluate the safety and efficacy of this gene therapy in patients with severe sickle cell disease and transfusion-dependent beta thalassemia.

What phase is EDIT-301 in?

EDIT-301 is in Phase 1 clinical development. It is an investigational therapy and has not been approved by regulatory authorities. The drug has received FDA designations including orphan drug, rare pediatric disease, and regenerative medicine advanced therapy (RMAT) designations.

What clinical trials is EDIT-301 in?

EDIT-301 is being studied in two Phase 1 clinical trials. The RUBY trial (NCT04853576) evaluates the therapy in patients with severe sickle cell disease, while a second trial (NCT05444894) is studying it in adults with transfusion-dependent beta thalassemia. Both trials are active but not recruiting participants.

Is EDIT-301 the same as a gene editing treatment for sickle cell disease?

EDIT-301 is a gene editing therapy specifically designed for sickle cell disease and beta thalassemia. It uses the AsCas12a enzyme to edit a patient's own stem cells ex vivo. The therapy is distinct from other gene therapies in its use of this particular enzyme and its approach to reactivating fetal hemoglobin.