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D-Fi

Phase 3

Dystrophic Epidermolysis Bullosa | Monoclonal antibody | Rare Disease |Castle Biosciences, Inc.|Last Updated: Mar 13, 2026

Success Probability

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Market & Valuation

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Trial Design

UNCONTROLLEDDMC
Total Trials1
Total Enrollment32

FDA Designations

No designations recorded

Clinical trial landscape

D-Fi · 1 trial · 1 indication

Phase 3 1
NCT06892639Evaluation of D-Fi for the Treatment of Wounds Due to DEBDystrophic Epidermolysis Bullosa
ACTIVE NOT_RECRUITING32 Analytics
PHASE3ACTIVE NOT_RECRUITING
Evaluation of D-Fi for the Treatment of Wounds Due to DEB
Dystrophic Epidermolysis BullosaUnlock trial analytics

Study Endpoints

Primary Endpoints

The difference in Complete (100%) Wound Closure of the Target Wound Pair at either Week 22 (Visit 6), and Week 24 (Visit 7) or at Week 24 (Visit 7) and Week 26 (Visit 8) as assessed by a Blinded Assessor.
Week 26
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Study Design & Arms

AllocationNA
MaskingNONE
ModelSINGLE_GROUP
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
D-Fi COL7A1 Genetically-Corrected Autologous FibroblastsEXPERIMENTALIntra-subject randomized (paired wounds in each subject receive experimental treatment, D-Fi, or remain untreated). One target wound pair will be identified for each subject. Following pairing, target wounds will be randomly assigned as the treatment wound (D-Fi is administered) or control wound. Subjects will receive intradermal injections of D-Fi in each specified treatment wound in two or more treatment sessions. The first treatment session occurs at Day 1, the second at Week 8/Month 2 and the third at Week 16/Month 4. Additional treatment sessions may occur at Week 26/Month 6, Week 32/Month 8 and Week 40/Month 10 when unclosed treatment wounds may be re-treated, and unclosed control wounds may be treated.

Interventions

NameTypeDescription
D-FiBIOLOGICALD-Fi is composed of fibroblasts isolated from the subject's skin biopsies which are genetically corrected with the full length COL7A1 gene encoding for type VII collagen.
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Eligibility Criteria

Age Range2 Years to N/A
SexALL
Healthy VolunteersNo
Study Sites4

Inclusion Criteria: * Male or female ≥2 years of age at the Screening visit * Clinical diagnosis of DEB with confirmation of COL7A1 genetic mutation. Exclusion Criteria: * Medical instability limiting ability to travel to the investigative site. * Active infection with human immunodeficiency viru...

Countries:United States
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Frequently asked questions about D-Fi

What is D-Fi used for?

D-Fi is an investigational monoclonal antibody being developed for the treatment of wounds due to dystrophic epidermolysis bullosa (DEB), a rare genetic skin disorder. It is currently in Phase 3 clinical development and has not been approved by the FDA.

Who is developing D-Fi?

D-Fi is being developed by Castle Biosciences, Inc. (NASDAQ: CSTL), a biopharmaceutical company focused on dermatologic diseases. The company is conducting a Phase 3 clinical trial of D-Fi in the United States.

What phase is D-Fi in?

D-Fi is in Phase 3 clinical development. It is an investigational drug and has not yet been approved by regulatory authorities. The ongoing Phase 3 trial is active but not recruiting participants.

What clinical trials is D-Fi in?

D-Fi is being evaluated in a Phase 3 clinical trial with the identifier NCT06892639, titled "Evaluation of D-Fi for the Treatment of Wounds Due to DEB." The trial is enrolling 32 participants aged 2 years and older in the United States.

How does D-Fi work?

D-Fi is a monoclonal antibody, a type of biologic drug designed to bind to a specific target involved in the disease process. Its exact molecular target has not been disclosed in available information.