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AUTO3 CD19/22 CAR T cells

Phase 1

B Acute Lymphoblastic Leukemia | Monoclonal antibody | Oncology |Autolus Therapeutics plc|Last Updated: Feb 1, 2021

Success Probability

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Trial Design

UNCONTROLLEDDMC
Total Trials1
Total Enrollment23

FDA Designations

No designations recorded

Clinical trial landscape

AUTO3 CD19/22 CAR T cells · 1 trial · 4 indications

Phase 1 1
NCT03289455CD19 /22 CAR T Cells (AUTO3) for the Treatment of B Cell Acute Lymphoblastic Leukemia (ALL)B Acute Lymphoblastic Leukemia
COMPLETED23 Analytics
PHASE1COMPLETED
CD19 /22 CAR T Cells (AUTO3) for the Treatment of B Cell Acute Lymphoblastic Leukemia (ALL)
B Acute Lymphoblastic LeukemiaUnlock trial analytics

Study Endpoints

Primary Endpoints

Number of Patients With Grade 3-5 Toxicities Occurring Within the Dose Limiting Toxicity (DLT) Period of AUTO3 Infusion
Within 30 days (+/- 3 days) after the last dose of AUTO3.
Number of Patients With Dose Limiting Toxicity (DLT) of AUTO3
Within 30 days (+/- 3 days) after the last dose of AUTO3.

DLT was defined as i) any new non-hematological adverse event (AE) of Grade 3 or higher toxicity using the NCI CTCAE (version 5.0), which was probably or definitely related to AUTO3 therapy, which occurred within the DLT evaluation period, and which failed to resolve to Grade 2 or better within 14 days, despite appropriate supportive measures; ii) Grade 4 cytokine release syndrome (CRS) or neurotoxicity, cerebral edema, or Grade 3 neurotoxicity (including cerebral edema) that lasted \>72 hours; iii) Grade \>3 disseminated intravascular coagulation; iv) Grade \>2 infusion reaction; v) Any other fatal event (Grade 5) or life-threatening event (Grade 4) that could not be managed with conventional supportive measures or which in the opinion of the Safety Evaluation Committee (SEC) necessitated dose reduction or other modification to trial treatment to avoid a similar hazard in future patients.

Number of Patients Achieving Morphological Remission (Complete Response(CR) or Complete Response With Incomplete Count Recovery (CRi) and Minimal Residual Disease (MRD)-Negative Response in the Bone Marrow (PCR)).
Within 30 days (+/- 3 days) post AUTO3 infusion

Morphological response evaluations were based on the response criteria for ALL according to the NCCN guidelines version 2.2014. Minimal residual disease-negative status was achieved if MRD was \<10\^-4 (0.01%) by PCR amplification of individual rearrangements of Ig genes and/or flow cytometry MRD testing.

Secondary Endpoints

Feasibility of Generating AUTO3: Number of Patients' Cells Successfully Manufactured as a Proportion of the Number of Patients Undergoing Leukapheresis
Up to 8 weeks post leukapheresis
Event-Free Survival (EFS) by Morphological Analysis
Up to 2 years
Number of Patients With CD19- and/or CD22-negative Relapse
Up to 2 years
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Study Design & Arms

AllocationNA
MaskingNONE
ModelSINGLE_GROUP
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
AUTO3EXPERIMENTALPaediatric patients with relapse or refractory B-cell ALL

Interventions

NameTypeDescription
AUTO3 (CD19/22 CAR T cellsBIOLOGICALFollowing preconditioning with chemotherapy (cyclophosphamide and fludarabine) patients will be treated with 1 to 5.0 x 10⁶/kg CD19/CD22 Chimeric Antigen Receptor (CAR) positive T cells as a single or split dose.
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Eligibility Criteria

Age Range1 Year to 24 Years
SexALL
Healthy VolunteersNo
Study Sites3

Key Inclusion Criteria: 1. Male or female patients aged 1-24 years with high risk (HR) relapsed/refractory B-lineage ALL, AND: 1. Any bone marrow (BM) relapse or central nervous system (CNS) relapse with detectable BM disease after allogeneic stem cell transplant (SCT) and must be ≥6 months fro...

Countries:United Kingdom
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Frequently asked questions about AUTO3 CD19/22 CAR T cells

What is AUTO3 CD19/22 CAR T cells used for?

AUTO3 CD19/22 CAR T cells is an investigational cell therapy being developed for the treatment of B Acute Lymphoblastic Leukemia (ALL), including recurrent and refractory childhood ALL. It is a bispecific CAR T cell product designed to target two antigens, CD19 and CD22, on B cells. It is currently in Phase 1 clinical development.

What does AUTO3 CD19/22 CAR T cells target?

AUTO3 CD19/22 CAR T cells targets both CD19 and CD22, two proteins found on B cells. By engineering a patient's T cells to recognize these targets, the therapy aims to direct the immune system to attack and eliminate cancerous B cells in B Acute Lymphoblastic Leukemia. This dual targeting is intended to improve treatment efficacy.

Who makes AUTO3 CD19/22 CAR T cells?

AUTO3 CD19/22 CAR T cells is being developed by Autolus Therapeutics plc, a biopharmaceutical company. The company's stock is traded under the ticker symbol AUTL. Autolus is focused on developing next-generation T cell therapies for the treatment of cancer, including this investigational CAR T cell product.

What phase is AUTO3 CD19/22 CAR T cells in?

AUTO3 CD19/22 CAR T cells is in Phase 1 clinical development. It is an investigational therapy, meaning it has not been approved by regulatory authorities and is still being studied in clinical trials. The Phase 1 trial has been completed, and the therapy is not yet available outside of clinical research settings.

What clinical trials is AUTO3 CD19/22 CAR T cells in?

AUTO3 CD19/22 CAR T cells has been studied in one clinical trial, identified as NCT03289455. This Phase 1 trial, titled "CD19/22 CAR T Cells (AUTO3) for the Treatment of B Cell Acute Lymphoblastic Leukemia (ALL)," was conducted in the United Kingdom and enrolled 23 participants. The trial has been completed.

Is AUTO3 CD19/22 CAR T cells the same as a CD19/22 CAR T cell therapy?

Yes, AUTO3 CD19/22 CAR T cells is also known as CD19/22 CAR T cells. The product is designed to target both CD19 and CD22 antigens, and it is being evaluated for the treatment of B Acute Lymphoblastic Leukemia. This dual-targeting approach distinguishes it from single-target CAR T cell therapies.