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ABI-4334

Phase 1

Chronic Hepatitis B | Small molecule | Infectious Disease |Assembly Biosciences, Inc.|Last Updated: Jul 23, 2025

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials2
Total Enrollment74

FDA Designations

No designations recorded

Clinical trial landscape

ABI-4334 · 2 trials · 1 indication

Phase 1 2
NCT06384131A Study to Assess the Safety, Pharmacokinetics, and Antiviral Activity of ABI-4334 in Subjects With Chronic Hepatitis B Virus InfectionChronic Hepatitis B
COMPLETED20 Analytics
NCT05569941A First in Human Study to Assess Safety, Tolerability, Pharmacokinetics of ABI-4334 in Healthy SubjectsChronic Hepatitis B
COMPLETED54 Analytics
PHASE1COMPLETED
A Study to Assess the Safety, Pharmacokinetics, and Antiviral Activity of ABI-4334 in Subjects With Chronic Hepatitis B Virus Infection
Chronic Hepatitis BUnlock trial analytics
PHASE1COMPLETED
A First in Human Study to Assess Safety, Tolerability, Pharmacokinetics of ABI-4334 in Healthy Subjects
Chronic Hepatitis BUnlock trial analytics

Study Endpoints

Primary Endpoints

To assess the safety and tolerability of ABI-4334 in subjects with cHBV following 28-day multiple oral doses
Through end of study, up to 56 days

Proportion of subjects with adverse events (AEs), premature treatment discontinuation due to AEs, and abnormal laboratory results

Proportion of subjects with adverse events (AEs), premature treatment discontinuation due to AEs, and abnormal laboratory results
Up to Day 14

Secondary Endpoints

To characterize the PK of ABI-4334 in plasma following 28-day multiple doses in subjects with cHBV
Through treatment period, up to 28 days
Changes in HBV DNA in subjects with cHBV following 28-day multiple doses of ABI-4334
Through treatment period, up to 28 days
Elimination half-life (t1/2) of ABI-4334 in subjects with cHBV
Through treatment period, up to 28 days
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Study Design & Arms

AllocationRANDOMIZED
MaskingTRIPLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
ABI-4334ACTIVE_COMPARATOR -
PlaceboPLACEBO_COMPARATOR -
Part A: SAD Cohorts 1-5 ABI-4334 TabletEXPERIMENTALA single dose of ABI-4334 will be administered on Day 1 in dose-escalation cohorts with a starting dose of 30 mg. The doses for subsequent cohorts will be determined by evaluation of safety and PK data from previous cohorts.
Part A: SAD Cohorts 1-5 ABI-4334 Placebo TabletPLACEBO_COMPARATORA single dose of placebo matching ABI-4334 will be administered on Day 1.
Part A: SAD Fed Cohorts 6-7 ABI-4334 TabletEXPERIMENTALA single dose of ABI-4334 will be administered after a high-fat meal on Day 1 in cohort 6. A single dose of ABI-4334 will be administered on two separate occasions, once fasted and once after a high-fat meal in cohort 7. The dose administered will be determined after evaluation of cumulative safety and PK data from cohorts 1-5.
Part A: SAD Fed Cohorts 6 ABI-4334 Placebo TabletPLACEBO_COMPARATORA single dose of placebo matching ABI-4334 will be administered on Day 1 after a high-fat meal on Day 1 in cohort 6.
Part B: MAD Cohorts 1-2 ABI-4334 TabletEXPERIMENTALOnce-daily doses of ABI-4334 will be administered from Day 1 to Day 8. Cohort B1 will receive a dose determined from evaluation of the data from the SAD cohorts. The doses for the subsequent cohort will be determined by evaluation of safety and PK data from previous cohorts.
Part B: MAD Cohorts 1-2 ABI-4334 Placebo TabletPLACEBO_COMPARATOROnce-daily doses of placebo matching ABI-4334 will be administered from Day 1 to Day 8.

Interventions

NameTypeDescription
ABI-4334DRUG10 mg or 50 mg tablets for oral administration
PlaceboDRUG10 mg or 50 mg tablets for oral administration
ABI-4334 TabletDRUGABI-4334 Tablet
ABI-4334 PlaceboDRUGPlacebo to ABI-4334 Tablet
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Eligibility Criteria

Age Range18 Years to 65 Years
SexALL
Healthy VolunteersNo
Study Sites2

Inclusion Criteria: 1. Body mass index (BMI) ≥ 18.0 and \< 35.0 kg/m(2), where BMI = weight (kg)/(height \[m\])(2) with a minimum body weight of 45 kg 2. Chronic hepatitis B infection, defined as HBV infection for ≥ 6 months documented 3. Treatment-naïve or off-antiviral therapy for ≥ 24 weeks prio...

Countries:MoldovaNew Zealand
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Frequently asked questions about ABI-4334

What is ABI-4334 used for?

ABI-4334 is an investigational small molecule being developed for the treatment of chronic Hepatitis B. It is currently in Phase 1 clinical development and has not been approved by the FDA. The drug is being studied for its safety, tolerability, pharmacokinetics, and antiviral activity in patients with chronic Hepatitis B virus infection.

Who makes ABI-4334?

ABI-4334 is being developed by Assembly Biosciences, Inc., a biopharmaceutical company traded on the NASDAQ under the ticker symbol ASMB. The company is conducting clinical trials to evaluate the drug's safety and efficacy in treating chronic Hepatitis B.

What phase is ABI-4334 in?

ABI-4334 is in Phase 1 clinical development. Two Phase 1 trials have been completed, one in healthy subjects and one in patients with chronic Hepatitis B. The drug is investigational and has not received FDA approval. It is still in the early stages of clinical testing.

What clinical trials is ABI-4334 in?

ABI-4334 has completed two Phase 1 clinical trials. NCT05569941 assessed safety, tolerability, and pharmacokinetics in 54 healthy subjects in New Zealand. NCT06384131 evaluated safety, pharmacokinetics, and antiviral activity in 20 patients with chronic Hepatitis B in Moldova and New Zealand. Both trials are completed.

How does ABI-4334 work?

The mechanism of action for ABI-4334 has not been disclosed in the available information. It is a small molecule being studied for the treatment of chronic Hepatitis B, but its specific molecular target has not been publicly detailed.