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Patisiran

Phase 3

TTR-mediated Amyloidosis | Small molecule | Rare Disease |Alnylam Pharmaceuticals, Inc.|Last Updated: Apr 20, 2026

Success Probability
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Trial Design
RandomizedDouble-BlindPLACEBO_CONTROLLEDDMC
Total Trials2
Total Enrollment254
FDA Designations
No designations recorded
Clinical trial landscape

Patisiran · 8 trials · 15 indications

Phase 3 5Phase 2 1Phase 1 1Early Phase 1 1
NCT03997383APOLLO-B: A Study to Evaluate Patisiran in Participants With Transthyretin Amyloidosis With Cardiomyopathy (ATTR Amyloidosis With Cardiomyopathy)Transthyretin Amyloidosis (ATTR) With Cardiomyopathy
COMPLETED360 Analytics
NCT03862807Patisiran in Patients With Hereditary Transthyretin-mediated Amyloidosis (hATTR Amyloidosis) Disease Progression Post-Liver TransplantAmyloidosis, Familial
COMPLETED24 Analytics
NCT03759379HELIOS-A: A Study of Vutrisiran (ALN-TTRSC02) in Patients With Hereditary Transthyretin Amyloidosis (hATTR Amyloidosis)Amyloidosis, Hereditary
COMPLETED164 Analytics
NCT02510261The Study of an Investigational Drug, Patisiran (ALN-TTR02), for the Treatment of Transthyretin (TTR)-Mediated Amyloidosis in Participants Who Have Already Been Treated With ALN-TTR02 (Patisiran)Amyloidosis
COMPLETED211 Analytics
NCT01960348APOLLO: The Study of an Investigational Drug, Patisiran (ALN-TTR02), for the Treatment of Transthyretin (TTR)-Mediated AmyloidosisTTR-mediated Amyloidosis
COMPLETED225 Analytics
PHASE3COMPLETED
APOLLO-B: A Study to Evaluate Patisiran in Participants With Transthyretin Amyloidosis With Cardiomyopathy (ATTR Amyloidosis With Cardiomyopathy)
Transthyretin Amyloidosis (ATTR) With CardiomyopathyUnlock trial analytics
PHASE3COMPLETED
Patisiran in Patients With Hereditary Transthyretin-mediated Amyloidosis (hATTR Amyloidosis) Disease Progression Post-Liver Transplant
Amyloidosis, FamilialUnlock trial analytics
PHASE3COMPLETED
HELIOS-A: A Study of Vutrisiran (ALN-TTRSC02) in Patients With Hereditary Transthyretin Amyloidosis (hATTR Amyloidosis)
Amyloidosis, HereditaryUnlock trial analytics
PHASE3COMPLETED
The Study of an Investigational Drug, Patisiran (ALN-TTR02), for the Treatment of Transthyretin (TTR)-Mediated Amyloidosis in Participants Who Have Already Been Treated With ALN-TTR02 (Patisiran)
AmyloidosisUnlock trial analytics
PHASE3COMPLETED
APOLLO: The Study of an Investigational Drug, Patisiran (ALN-TTR02), for the Treatment of Transthyretin (TTR)-Mediated Amyloidosis
TTR-mediated AmyloidosisUnlock trial analytics
Study Endpoints
Primary Endpoints
Change From Baseline at Month 12 in Six-Minute Walk Test (6-MWT)
Baseline, Month 12

Distance in meters walked in 6 minutes, longer distances indicate greater functional capacity. Missing 6MWT values due to non-COVID-19 death or inability to walk due to ATTR disease progression were imputed using the worst 10th percentile change observed in the DB period. Missing 6-MWT values due to other reasons are multiply imputed to create 100 complete datasets. The change from baseline is averaged across the 100 complete datasets.

Average of Month 6 and Month 12 Percentage Reduction From Baseline in Serum Transthyretin (TTR)
Baseline, Months 6 and 12

Serum TTR was assessed using enzyme linked immunosorbent assay (ELISA). The average of the percentage reduction in serum TTR observed at Month 6 and at Month 12 is first calculated for each patient and then the median (95% CI) of these averaged values is summarized for the Safety Analysis Set.

Change From Baseline in the Modified Neurologic Impairment Score +7 (mNIS+7) at Month 9 Between the Vutrisiran Group (HELIOS-A) and the External Placebo Comparator Group [APOLLO (NCT01960348)]
Baseline, Month 9

The mNIS+7 is a composite score that measures neurologic impairment which includes the following components: physical exam of lower limbs, upper limbs and cranial nerves to assess motor strength/weakness, electrophysiologic measurement of small and large nerve fiber function, sensory testing and postural blood pressure. The mNIS+7 is scored from 0 (no impairment) to 304 points (maximum impairment). A higher score indicates a worse outcome.

Percentage of Participants With Adverse Events (AEs) Leading to Study Discontinuation
First dose up to 28 days after last dose of study drug (approximately 5.6 years)

AE is any untoward medical occurrence in a participant or clinical investigational subject administered a medicinal product and which does not necessarily have a causal relationship with this treatment.

Modified Neuropathy Impairment Score +7 (mNIS+7)
18mo

The difference between the patisiran (ALN-TTR02) and placebo groups in the change from baseline in mNIS+7 at 18 months. The mNIS+7 is a composite score that quantitates motor, sensory, and autonomic neurologic impairment due to injury of large and small nerves. The minimum and maximum values are 0 and 304, respectively. A higher score indicates a worse outcome.

Number of Participants With Adverse Events (AEs), Serious Adverse Events (SAEs) and Study Drug Discontinuation
Up to 56 days post first dose

The number of participants experiencing at least one adverse event (AE), at least one serious adverse event (SAE) and study drug discontinuation (due to any reason).

The proportion of subjects experiencing adverse events (AEs), serious adverse events (SAEs) and study drug discontinuation
Up to 28 days
Change in Neurological Impairment Score
baseline to 24 months

to assess the severity of functional impairment of motor and sensory nerves.NIS is a measure of motor strength, comprised of cranial nerve and both upper and lower limb motor assessments. The minimum and maximum values are 0 and 192, respectively. A higher score indicates a worse outcome.

Norfolk QOL-DN
baseline to 24 months

Change in Norfolk Quality of Life Questionnaire (Norfolk QOL-DN).The change from baseline in Norfolk QoL-DN at 24 months. The Norfolk QoL-DN questionnaire is a standardized 35-item patient-reported outcomes measure of diabetic neuropathy - small fiber, large fiber, and autonomic nerve function. The minimum and maximum values are -4 and 136, respectively. A higher score indicates a worse outcome.

COMPASS 31score
baseline to 24 months

Composite Autonomic Symptom Score (COMPASS) 31a self-assessment instrument for patient reported autonomic symptoms such as dizziness, constipation, diarrhea, nausea/vomiting, and incontinence. The minimum and maximum values are 0 and 100, respectively. A higher score indicates a worse outcome

Secondary Endpoints
Change From Baseline at Month 12 in Kansas City Cardiomyopathy Questionnaire Overall Summary (KCCQ-OS) Score
Baseline, Month 12
Composite Endpoint of All-Cause Mortality, Frequency of Cardiovascular (CV) Events (CV Hospitalizations and Urgent Heart Failure [HF] Visits) and Change From Baseline in 6-MWT Analyzed by Win Ratio
Up to Month 12
Composite Endpoint of All-Cause Mortality and Frequency of All-Cause Hospitalizations and Urgent HF Visits in Participants Not on Tafamidis at Baseline
Up to Month 12
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Study Design & Arms
AllocationRANDOMIZED
MaskingQUADRUPLE
ModelPARALLEL
PurposeTREATMENT
Treatment Arms
ArmTypeDescription
PatisiranEXPERIMENTALParticipants will be administered multiple doses of patisiran in the double-blind and open-label extension period.
PlaceboPLACEBO_COMPARATORParticipants will be administered multiple doses of placebo in the double-blind period. In the open-label extension period, participants will be administered multiple doses of patisiran.
Vutrisiran + Vutrisiran (HELIOS-A)EXPERIMENTALParticipants will receive vutrisiran 25 mg subcutaneous (SC) injection once every 3 months (q3M) for 18 months during the Treatment Period followed by vutrisiran 50 mg SC injection once every 6 months (q6M) or vutrisiran 25 mg q3M during the Randomized Treatment Extension (RTE) Period. Upon implementation of Amendment 6, participants receiving vutrisiran SC 50 mg q6M will transition to vutrisiran SC 25 mg q3M at their next scheduled dosing.
Patisiran + Vutrisiran (HELIOS-A)ACTIVE_COMPARATORParticipants will receive patisiran 0.3 mg/kg intravenous (IV) infusion once every 3 weeks (q3w) for 18 months during the Treatment Period followed by vutrisiran 50 mg SC injection once q6M or vutrisiran 25 mg q3M during the RTE Period. Upon implementation of Amendment 6, participants receiving vutrisiran SC 50 mg q6M will transition to vutrisiran SC 25 mg q3M at their next scheduled dosing.
Prior Placebo Group of Study 004EXPERIMENTALParticipants who received placebo and completed parent study ALN-TTR02-004 (NCT01960348) were enrolled to receive 0.3 milligrams per kilogram (mg/kg) patisiran intravenously (IV) once every 3 weeks (Q3W) up to 65.5 months.
Prior Patisiran Group of Study 004EXPERIMENTALParticipants who received patisiran and completed parent study ALN-TTR02-004 (NCT01960348) were enrolled to receive 0.3 mg/kg patisiran IV Q3W up to 66.9 months.
Prior Patisiran Group of Study 003EXPERIMENTALParticipants who received patisiran and completed parent study ALN-TTR02-003 (NCT01961921) were enrolled to receive 0.3 mg/kg patisiran IV Q3W up to 61.4 months.
patisiran (ALN-TTR02)ACTIVE_COMPARATOR -
Sterile Normal Saline (0.9% NaCl)PLACEBO_COMPARATOR -
open labelOTHERsingle open arm label
Interventions
NameTypeDescription
PlaceboDRUGNormal saline (0.9% NaCl) matching volume of patisiran doses will be administered intravenously.
PatisiranDRUGPatisiran will be administered by intravenous (IV) infusion.
VutrisiranDRUGVutrisiran will be administered by SC injection.
patisiran (ALN-TTR02)DRUGadministered by intravenous (IV) infusion
Sterile Normal Saline (0.9% NaCl)DRUGadministered by intravenous (IV) infusion
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Eligibility Criteria
Age Range18 Years to 85 Years
SexALL
Healthy VolunteersNo
Study Sites90

Inclusion Criteria: * Documented diagnosis of ATTR amyloidosis with cardiomyopathy, classified as either hereditary ATTR amyloidosis with cardiomyopathy or wild-type ATTR amyloidosis with cardiomyopathy * Medical history of heart failure with at least 1 prior hospitalization for heart failure, or c...

Countries:United StatesArgentinaAustraliaBelgiumBrazilBulgariaChileCzechiaDenmarkFranceHong KongItalyJapanMexicoNetherlandsNew ZealandPolandPortugalSouth KoreaSwedenTaiwanUnited KingdomGermanySpainCanadaCyprusGreeceMalaysiaTurkey (Türkiye)
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Recent Changes (Last 90 Days)
MEDIUMMay 26, 2026NCT03997383TRIAL_REMOVED: changed
LOWMay 24, 2026NCT05023889studyFirstPostDate: changed
LOWMay 24, 2026NCT03997383studyFirstPostDate: changed