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ABT-333

Phase 2

Chronic Hepatitis C Virus Infection | Small molecule | Infectious Disease |AbbVie Inc.|Last Updated: Jul 2, 2018

Success Probability

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Market & Valuation

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Trial Design

RandomizedDouble-BlindPLACEBO_CONTROLLED
Total Trials1
Total Enrollment30

FDA Designations

No designations recorded

Clinical trial landscape

ABT-333 · 4 trials · 3 indications

Phase 2 2Phase 1 2
NCT00851890A Study to Evaluate the Safety, Tolerability, Pharmacokinetics, and Antiviral Activity of Multiple Doses of ABT-333 Alone and in Combination With Pegylated Interferon (pegIFN) and Ribavirin (RBV) in Subjects With Genotype 1 Chronic Hepatitis C Virus (HCV) InfectionChronic Hepatitis C Virus Infection
COMPLETED30 Analytics
NCT00726882A Follow-up Assessment of Resistance to ABT-333 in Hepatitis C Virus (HCV)-Infected Subjects Who Have Received ABT-333 in ABT-333 StudiesHCV Infection
COMPLETED35 Analytics
PHASE2COMPLETED
A Study to Evaluate the Safety, Tolerability, Pharmacokinetics, and Antiviral Activity of Multiple Doses of ABT-333 Alone and in Combination With Pegylated Interferon (pegIFN) and Ribavirin (RBV) in Subjects With Genotype 1 Chronic Hepatitis C Virus (HCV) Infection
Chronic Hepatitis C Virus InfectionUnlock trial analytics
PHASE2COMPLETED
A Follow-up Assessment of Resistance to ABT-333 in Hepatitis C Virus (HCV)-Infected Subjects Who Have Received ABT-333 in ABT-333 Studies
HCV InfectionUnlock trial analytics

Study Endpoints

Primary Endpoints

Mean Maximal Change From Baseline in Hepatitis C Virus Ribonucleic Acid (HCV RNA) Levels During ABT-333 Monotherapy Treatment
Prior to the first dose on Day 1 to before first dose on Day 3

Serum hepatitis C virus ribonucleic acid (HCV RNA) levels (reported as log10 IU/mL) were determined for each sample using a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay. The baseline value was the HCV RNA measurement before the first dose of ABT-333 on Day 1. The maximal change during monotherapy was nadir minus the baseline log10 HCV RNA level. Nadir was defined as the lowest log10 HCV RNA level any time after the first dose of study drug on Day 1 through the last log10 HCV RNA level before the first dose of study drug on Day 3. Data are reported as the least squares mean change from nadir ± standard error.

Mean Maximal Change From Baseline in Hepatitis C Virus Ribonucleic Acid (HCV RNA) Levels Through Day 28
Prior to the first dose on Day 1 through Day 28

Serum hepatitis C virus ribonucleic acid (HCV RNA) levels (reported as log10 IU/mL) were determined for each sample using a real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay. The baseline value was the HCV RNA measurement before the first dose of ABT-333 on Day 1. The maximal change during treatment was nadir minus the baseline log10 HCV RNA level. Nadir was defined as the lowest log10 HCV RNA level any time after the first dose of study drug on Day 1 through the last log10 HCV RNA level on Day 28. Data are reported as the least squares mean change from nadir ± standard error.

Maximum Plasma Concentration (Cmax) of ABT-333
Pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours post-dose on Day 1; and pre-dose on Day 2

Blood samples were collected pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours after the morning dose on Day 1; and pre-dose on Day 2. The samples were analyzed for the concentration of ABT-333 using validated analytical methods. The maximum plasma concentration (Cmax; measured in ng/mL) is the highest concentration that a drug achieves in the blood after administration in a dosing interval. The Cmax of ABT-333 was estimated using non-compartmental methods and data are reported as the mean ± standard deviation.

Time to Maximum Plasma Concentration (Tmax) of ABT-333
Pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours post-dose on Day 1; and pre-dose on Day 2

Blood samples were collected pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours after the morning dose on Day 1; and pre-dose on Day 2. The samples were analyzed for the concentration of ABT-333 using validated analytical methods. The time to maximum plasma concentration (Tmax; measured in hours) is the time it takes for a drug to achieve Cmax. The Tmax of ABT-333 was estimated using non-compartmental methods and data are reported as the mean ± standard deviation.

Area Under the Plasma Concentration-time Curve From 0 to 12 Hours Post-dose (AUC12) of ABT-333
Pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours post-dose on Day 1; and pre-dose on Day 2

Blood samples were collected pre-dose (time 0 hours); 2, 4, 8, 12, and 16 hours after the morning dose on Day 1; and pre-dose on Day 2. The samples were analyzed for the concentration of ABT-333 using validated analytical methods. The area under the plasma concentration-time curve (AUC; measured in ng\*hr/mL) measures the total exposure of a drug in blood plasma. The AUC12 of ABT-333 was estimated using non-compartmental methods and data are reported as the mean ± standard deviation.

Serum Concentrations of Pegylated Interferon (pegIFN)
Prior to the morning dose on Day 3; 4 hours after the morning dose on Day 3; prior to the morning dose on Days 4 and 5; and single samples were collected on Days 10, 17, 24, and 28

Blood samples were collected prior to the morning dose on Day 3; 4 hours after the morning dose on Day 3; prior to the morning dose on Days 4 and 5; and single samples were collected on Days 10, 17, 24, and 28. The samples were analyzed for the concentration of pegIFN (measured in ng/mL) using validated analytical methods and estimated using non-compartmental methods. Data are reported as the mean ± standard deviation.

Plasma Concentrations of Ribavirin (RBV)
Prior to the morning dose on Day 3; 4 hours after the morning dose on Day 3; prior to the morning dose on Days 4 and 5; and single samples were collected on Days 10, 17, 24, and 28

Blood samples were collected prior to the morning dose on Day 3; 4 hours after the morning dose on Day 3; prior to the morning dose on Days 4 and 5; and single samples were collected on Days 10, 17, 24, and 28. The samples were analyzed for the concentration of RBV (measured in ng/mL) using validated analytical methods and estimated using non-compartmental methods. Data are reported as the mean ± standard deviation.

Number of Participants Having Treatment-emergent Adverse Events (AEs)
AEs were collected from the time of study drug administration to 30 days after last dose of study drug (8 Weeks)

An AE was any untoward medical occurrence that did not have a causal relationship with treatment. An Adverse Drug Reaction (ADR) was any noxious and undesired reaction related to the experimental drug or experiment. A serious adverse event (SAE) was an AE that resulted in death, was life-threatening, resulted in or prolonged hospitalization, resulted in congenital anomaly, was persistent or caused significant disability/incapacity, spontaneous or elective abortion, or required intervention to prevent a serious outcome. AEs were rated for severity as either: 1. Mild - transient and easily tolerated; 2. Moderate - caused discomfort and interrupted usual activities; 3. Severe - caused considerable interference with usual activities, may be incapacitating or life-threatening. AEs related to direct-acting antiviral agents (DAAs) were assessed as being either probably or possibly related by the investigator.

Persistence of Resistance-Associated Variants and Phenotypic Resistance
Baseline (day of study completion or early discontinuation from the prior ABT-333 clinical study), 48 weeks

Participants in studies M10-351 (NCT00851890) and M10-380 (NCT00696904) were analyzed for persistence of resistance-associated variants by comparing post-treatment clonal sequence data with baseline and on-treatment sequence data from M10-351 and M10-380 studies to assess amino acid changes. Phenotypic resistance to ABT-333 was assessed by calculating the fold change in half maximal effective concentration (EC50) of post-treatment samples compared with the EC50 value for the corresponding baseline sample as determined for M10-351 and M10-380 studies. The number of participants with variants at resistance-associated amino acid positions and phenotypic resistance at post-treatment time points are presented. Variants are included if the absolute percent of total clones encoding the variant was at least 10% greater than at baseline in a post-treatment sample.

ABT-333 drug concentrations
Day 1 until 24 hours after single dose of ABT-333 for each period

ABT-333 concentrations in blood

Analysis of pharmacokinetic variables and mean change in HCV RNA level from baseline.
approximately 1 week or less
Analysis of safety measures, including but not limited to tabulation of adverse events, physical exam, clinical lab results (include chemistry, hematology and urine) and vital signs.
approximately 1 week

Secondary Endpoints

Change From Baseline in Hepatitis C Virus Ribonucleic Acid (HCV RNA) Levels Through Day 28 or Final Visit
Day 28 and Final Visit
Percentage of Participants With at Least a 2 log10 Maximal Decrease in Hepatitis C Virus Ribonucleic Acid (HCV RNA) Levels During ABT-333 Treatment
Prior to the first dose on Day 1 and Day 28
Percentage of Participants With Hepatitis C Virus Ribonucleic Acid (HCV RNA) Levels ≤ 25 IU/mL (the Lower Limit of Quantitation [LLOQ]) at Day 28 or Final Visit
Day 28 or Final Visit
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Study Design & Arms

AllocationRANDOMIZED
MaskingDOUBLE
ModelPARALLEL
PurposeTREATMENT

Treatment Arms

ArmTypeDescription
ABT-333 (300 mg) twice daily (BID) + pegIFN/RBVEXPERIMENTALHepatitis C virus (HCV) positive, treatment-naive participants received 300 mg ABT-333 BID for 2 days followed by 300 mg ABT-333 BID with pegylated interferon/ribavirin (pegIFN/RBV) for 26 days. Pegylated interferon was administered at 180 μg subcutaneously once a week and RBV was dosed 1000 or 1200 mg daily divided twice a day.
ABT-333 (600 mg) twice daily (BID) + pegIFN/RBVEXPERIMENTALHepatitis C virus (HCV) positive, treatment-naive participants received 600 mg ABT-333 BID for 2 days followed by 600 mg ABT-333 BID with pegylated interferon/ribavirin (pegIFN/RBV) for 26 days. Pegylated interferon was administered at 180 μg subcutaneously once a week and RBV was dosed 1000 or 1200 mg daily divided twice a day.
ABT-333 (1200 mg) once daily (QD) + pegIFN/RBVEXPERIMENTALHepatitis C virus (HCV) positive, treatment-naive participants received 1200 mg ABT-333 QD for 2 days followed by 1200 mg ABT-333 QD with pegylated interferon/ribavirin (pegIFN/RBV) for 26 days. Pegylated interferon was administered at 180 μg subcutaneously once a week and RBV was dosed 1000 or 1200 mg daily divided twice a day.
Placebo + pegIFN/RBVPLACEBO_COMPARATORHepatitis C virus (HCV) positive, treatment-naïve participants received matching placebo once daily (QD) or twice daily (BID) for 2 days followed by placebo QD or BID with pegylated interferon/ribavirin (pegIFN/RBV) for 26 days. Pegylated interferon was administered at 180 μg subcutaneously once a week and RBV was dosed 1000 or 1200 mg daily divided twice a day.
HCV-infected ParticipantsOTHERHepatitis C virus (HCV)-infected participants who received ABT-333 at any dose level or matching placebo in a prior clinical study involving ABT-333. Participants received no treatment in this follow-up study.
Arm 1: ABT-333EXPERIMENTALA single centre, open-label, 4-treatment, 3-period, 4-sequence incomplete randomised, single dose crossover study in healthy subjects.
1OTHERHealthy volunteers, receiving 10-1200 mg ABT-333 or placebo, single dose
2OTHERHCV+ treatment-naive subjects receiving 100-300 mg ABT-333 or placebo, multi-dose, QD or BID
3OTHERHealthy volunteers, receiving 100 mg ABT-333, multi-dose, food effect

Interventions

NameTypeDescription
ABT-333DRUG50 mg capsules
Placebo for ABT-333OTHERCapsule
Pegylated interferonDRUGSyringe, 180 µg/0.5 mL for subcutaneous injections administered weekly
RibavirinDRUG200 mg tablet dosed at 1000 or 1200 mg daily divided twice a day
Blood sample collection onlyPROCEDUREApproximately monthly collection of blood samples.
PlaceboDRUGCapsule, see arms for intervention description
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Eligibility Criteria

Age Range18 Years to 70 Years
SexALL
Healthy VolunteersNo
Study Sites8

Inclusion Criteria: * Participant has provided written consent. * If female, participant is postmenopausal or surgically sterile. * If male, must be practicing two effective methods of birth control. * Participant is hepatitis C virus (HCV) genotype 1 with HCV ribonucleic acid levels \>50,000 IU/mL...

Countries:United StatesPuerto RicoUnited Kingdom
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Frequently asked questions about ABT-333

What is ABT-333 used for?

ABT-333 is an investigational small molecule being developed for chronic Hepatitis C Virus (HCV) infection. It has been studied in clinical trials for HCV infection, including genotype 1 chronic hepatitis C, and for relative bioavailability assessment in healthy subjects. It is not approved and remains in clinical development.

What does ABT-333 target?

ABT-333 is a small molecule antiviral agent studied for the treatment of Hepatitis C Virus (HCV) infection. It has been evaluated in combination with pegylated interferon and ribavirin in clinical trials. The specific molecular target is not disclosed in the available data.

Who is developing ABT-333?

ABT-333 is being developed by AbbVie Inc., a biopharmaceutical company listed on the New York Stock Exchange under the ticker ABBV. The company has sponsored clinical trials evaluating the drug for chronic Hepatitis C Virus infection and related conditions.

What phase is ABT-333 in?

ABT-333 has completed Phase 1 and Phase 2 clinical trials. The most advanced trials are Phase 2 studies, which have been completed. The drug is investigational and has not received FDA approval. No active trials are currently listed for ABT-333.

What clinical trials is ABT-333 in?

ABT-333 has been studied in several completed clinical trials, including NCT00696904, a Phase 1 study in healthy volunteers and HCV-infected subjects; NCT00726882, a Phase 2 follow-up resistance assessment; NCT00851890, a Phase 2 study of ABT-333 alone and with pegylated interferon and ribavirin; and NCT02052349, a Phase 1 bioavailability study.

Is ABT-333 the same as dasabuvir?

ABT-333 is also known as dasabuvir, an antiviral drug used in combination therapies for chronic Hepatitis C Virus infection. It was developed by AbbVie and has been evaluated in clinical trials for HCV genotype 1 infection. The drug is not FDA approved as a standalone therapy.